Name: Cell Meter™ Fluorimetric Phagocytosis Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 21225
Price: 448 USD
Availability: InStock

Cell Meter™ Fluorimetric Phagocytosis Assay Kit

Red Fluorescence

Cell Meter™ Fluorimetric Phagocytosis Assay Kit enables sensitive red-fluorescence-based detection of phagocytic activity in live cells.

pH-activated fluorescence detection: Uses Protonex™ Red 600-latex beads that fluoresce only upon acidification in phagosomes
Dual-color assay format: Includes a green viability dye to concurrently assess live cells and phagocytic function
Wide application range: Ideal for studying innate immune responses, drug screening, and cell-based functional assays
Comparable alternative: Provides a dual-fluorescence alternative to BioLegend’s Phagocytosis Detection Kit

<p>Examination of phagocytosis in RAW 264.7 cells using Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Cat# 21225). RAW 264.7 cells were incubated with (B) or without (A) Cytochalasin D for 30 min before Protonex™ 600 Red-Latex Beads in growth medium was added and incubated for 4 hours before Cell Tracker was added and incubated for 30 minutes. The images were taken using Keyence Fluorescence microscopy. </p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21225	100 Tests	Price

Overview

The Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Red Fluorescence) is designed to quantify phagocytic activity in live cells using a pH-activated fluorescence readout. It employs Protonex™ Red 600-latex bead conjugates, which are non-fluorescent at neutral pH but become brightly fluorescent upon acidification within phagosomes and phagolysosomes. This eliminates the need for a trypan blue quenching step and enables precise monitoring of particle internalization and phagosome maturation. The kit includes a green fluorescent viability dye for dual-color analysis, allowing simultaneous assessment of live cells and phagocytosis. Compatible with fluorescence microscopy, flow cytometry, and plate readers, it offers a flexible and high-throughput solution for studying innate immune responses, cell function, and drug effects on phagocytosis.

Citations

View all 6 citations: Citation Explorer

Sex-specific activation of platelet purinergic signaling is key in local cytokine release and phagocytosis in the peritoneal cavity in intra-abdominal sepsis

Authors:

Entsie, Philomena and Amoafo, Emmanuel Boadi and Kang, Ying and Gustad, Thomas and Dorsam, Glenn P and Frey, Mark R and Liverani, Elisabetta

Journal:

American Journal of Physiology-Cell Physiology (2025)

An integrated investigation of mitochondrial genes in COPD reveals the causal effect of NDUFS2 by regulating pulmonary macrophages

Authors:

Zou, Xiaoli and Huang, Qiqing and Kang, Tutu and Shen, Shaoran and Cao, Chenxi and Wu, Jianqing

Journal:

Biology Direct (2025): 4

Methylsulfonylmethane protects against lethal dose MRSA-induced sepsis through promoting M2 macrophage polarization

Authors:

Ma, Wei and Ao, Shengxiang and Zhou, Jianping and Li, Jiaxin and Liang, Xin and Yang, Xue and Zhang, Hao and Liu, Boyang and Tang, Wanqi and Liu, Haoru and others,

Journal:

Molecular Immunology (2022): 69--77

The N 6-methyladenosine (m6A)-forming enzyme METTL3 facilitates M1 macrophage polarization through the methylation of STAT1 mRNA

Authors:

Liu, Yihan and Liu, Zhujiang and Tang, Hao and Shen, Yicong and Gong, Ze and Xie, Nan and Zhang, Xu and Wang, Wengong and Kong, Wei and Zhou, Yuan and others,

Journal:

American Journal of Physiology-Cell Physiology (2019): C762--C775

The N6-Methyladenosine (m6A)-Forming Enzyme METTL3 Facilitates M1 Macrophage Polarization through the Methylation of STAT1 mRNA

Authors:

Liu, Yihan and Liu, Zhujiang and Tang, Hao and Shen, Yicong and Gong, Ze and Xie, Nan and Zhang, Xu and Wang, Wengong and Kong, Wei and Zhou, Yuan and others, undefined

Journal:

American Journal of Physiology-Cell Physiology (2019)

References

View all 56 references: Citation Explorer

Monitoring phospholipid dynamics during phagocytosis: application of genetically-encoded fluorescent probes

Authors:

Sarantis H, Grinstein S.

Journal:

Methods Cell Biol (2012): 429

Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock

Authors:

Schreiner L, Huber-Lang M, Weiss ME, Hohmann H, Schmolz M, Schneider EM.

Journal:

J Cell Commun Signal (2011): 135

The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis

Authors:

Flannagan RS, Grinstein S.

Journal:

Methods Mol Biol (2010): 121

Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms

Authors:

Leclerc L, Boudard D, Pourchez J, Forest V, Sabido O, Bin V, Palle S, Grosseau P, Bernache D, Cottier M.

Journal:

Inhal Toxicol (2010): 1091

Analysis of macrophage phagocytosis: quantitative assays of phagosome formation and maturation using high-throughput fluorescence microscopy

Authors:

Steinberg BE, Grinstein S.

Journal:

Methods Mol Biol (2009): 45

Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)	576
Emission (nm)	597

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Texas Red/FITC filter

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025

Component A: Protonex™ Red 600-Latex Bead Conjugates	1 vial (15 µL)
Component B: CytoTrace™ Green	1 vial
Component C: DMSO	1 vial (100 µL)