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Cyanine-3- dUTP [Cy3-dUTP] *1 mM in Tris Buffer (pH 7.5)*

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Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.
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InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1150.00
Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy3-dUTP conjugate can be used as a red fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis). It is functionally equivalent to Cyanine 3-dUTP (Perkin Elmer’s NEL578001EA).


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cyanine-3- dUTP [Cy3-dUTP] *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM86.957 µL434.783 µL869.565 µL4.348 mL8.696 mL
5 mM17.391 µL86.957 µL173.913 µL869.565 µL1.739 mL
10 mM8.696 µL43.478 µL86.957 µL434.783 µL869.565 µL

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Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cyanine 3 bisacid [equivalent to Cy3® bisacid]55556915000010.1510.070.073
Cyanine 3 monoacid [equivalent to Cy3® acid]55556915000010.1510.070.073
Cyanine 3 maleimide [equivalent to Cy3® maleimide]55556915000010.1510.070.073
Cyanine 3 azide [equivalent to Cy3® azide]55556915000010.1510.070.073
Cyanine 3 alkyne [equivalent to Cy3® alkyne]55556915000010.1510.070.073
Cyanine 3 amine [equivalent to Cy3® amine]55556915000010.1510.070.073
Cyanine 3 hydrazide [equivalent to Cy3® hydrazide]55556915000010.1510.070.073
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in Tris Buffer (pH 7.5)*65167025000010.271, 0.420.020.03



View all 48 references: Citation Explorer
A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
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Journal: Journal of agricultural and food chemistry (2020): 369-375
Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77
Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
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Journal: Analytical chemistry (2012): 5645-52
Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
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Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
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Journal: Animal reproduction science (2011): 32-6
Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94
[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5
A technical note on quantum dots for multi-color fluorescence in situ hybridization.
Authors: Müller, S and Cremer, M and Neusser, M and Grasser, F and Cremer, T
Journal: Cytogenetic and genome research (2009): 351-9
[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603
[Effect of bicyclol on gene expression profiles in mice with liver injury induced by concanavalin A].
Authors: Yu, Ying-Nan and Guo, Jiang and Li, Ye and Hong, Yuan and Li, Kang and Cheng, Jun and Li, Yan
Journal: Yao xue xue bao = Acta pharmaceutica Sinica (2008): 596-600