D-Luciferin galactoside CAS 131474-38-9

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Physical properties

Molecular weight	442.46
Solvent	DMSO

Spectral properties

Excitation (nm)	335
Emission (nm)	530

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41105331

Overview SDS Protocol

See also: Enzymes, Luciferases, Oxidases

CAS

131474-38-9

Molecular weight

442.46

Excitation (nm)

335

Emission (nm)

530

D-Luciferin galactoside contains a beta-galactoside moiety attached at the 6-O-position, thus preventing it be recognized by the firefly luciferase enzyme. However, the galactose moiety is removed effectively by beta-galactosidase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin galactoside is a sensitive substrate for the chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors. When used as a dual substrate, beta-galactosidase (LacZ gene) and luciferase levels can be determined with high sensitivity. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. It can also be used for in vivo bioluminescence imaging applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin galactoside *CAS 131474-38-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	226.009 µL	1.13 mL	2.26 mL	11.3 mL	22.601 mL
5 mM	45.202 µL	226.009 µL	452.018 µL	2.26 mL	4.52 mL
10 mM	22.601 µL	113.005 µL	226.009 µL	1.13 mL	2.26 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	335
Emission (nm)	530

Product Family

Name	Excitation (nm)	Emission (nm)
D-Luciferin phosphate CAS 145613-12-3	335	530
D-Luciferin acetate	335	530

Images

Figure 1. Chemical structure for D-Luciferin galactoside *CAS 131474-38-9*

Citations

View all 2 citations: Citation Explorer

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9

Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

References

View all 3 references: Citation Explorer

Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
Authors: Wehrman TS, von Degenfeld G, Krutzik PO, Nolan GP, Blau HM.
Journal: Nat Methods (2006): 295

Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors
Authors: Yang X, Janatova J, Andrade JD.
Journal: Anal Biochem (2005): 102

Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay
Authors: Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.
Journal: Lett Appl Microbiol (2000): 130