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D-Luciferin galactoside *CAS 131474-38-9*

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Physical properties
Molecular weight442.46
Spectral properties
Excitation (nm)385
Emission (nm)529
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
D-Luciferin galactoside contains a beta-galactoside moiety attached at the 6-O-position, thus preventing it be recognized by the firefly luciferase enzyme. However, the galactose moiety is removed effectively by beta-galactosidase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin galactoside is a sensitive substrate for the chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors. When used as a dual substrate, beta-galactosidase (LacZ gene) and luciferase levels can be determined with high sensitivity. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. It can also be used for in vivo bioluminescence imaging applications.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin galactoside *CAS 131474-38-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM226.009 µL1.13 mL2.26 mL11.3 mL22.601 mL
5 mM45.202 µL226.009 µL452.018 µL2.26 mL4.52 mL
10 mM22.601 µL113.005 µL226.009 µL1.13 mL2.26 mL

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Spectral properties

Excitation (nm)385
Emission (nm)529

Product Family

NameExcitation (nm)Emission (nm)
D-Luciferin phosphate *CAS 145613-12-3*385529
D-Luciferin acetate385529



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View all 3 references: Citation Explorer
Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence
Authors: Wehrman TS, von Degenfeld G, Krutzik PO, Nolan GP, Blau HM.
Journal: Nat Methods (2006): 295
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Authors: Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.
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