D-Luciferin | AAT Bioquest

D-Luciferin galactoside

CAS 131474-38-9

D-Luciferin galactoside contains a beta-galactoside moiety attached at the 6-O-position, thus preventing it be recognized by the firefly luciferase enzyme. However, the galactose moiety is removed effectively by beta-galactosidase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin galactoside is a sensitive substrate for the chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors. When used as a dual substrate, beta-galactosidase (LacZ gene) and luciferase levels can be determined with high sensitivity. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. It can also be used for in vivo bioluminescence imaging applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12513	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin galactoside *CAS 131474-38-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	226.009 µL	1.13 mL	2.26 mL	11.3 mL	22.601 mL
5 mM	45.202 µL	226.009 µL	452.018 µL	2.26 mL	4.52 mL
10 mM	22.601 µL	113.005 µL	226.009 µL	1.13 mL	2.26 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	442.46 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity

Authors:

Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan

Journal:

Journal of Extracellular Vesicles (2024): e12490

Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus

Authors:

Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco

Journal:

Proceedings of the National Academy of Sciences (2024): e2316646121

GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs

Authors:

Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,

Journal:

Plant Physiology (2023): kiad445

Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea

Authors:

Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,

Journal:

Bioorganic Chemistry (2023): 106625

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition

Authors:

Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan

Journal:

Oncogenesis (2022): 1--9

References

View all 3 references: Citation Explorer

Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence

Authors:

Wehrman TS, von Degenfeld G, Krutzik PO, Nolan GP, Blau HM.

Journal:

Nat Methods (2006): 295

Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors

Authors:

Yang X, Janatova J, Andrade JD.

Journal:

Anal Biochem (2005): 102

Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay

Authors:

Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.

Journal:

Lett Appl Microbiol (2000): 130

Physical properties

Molecular weight	442.46
Solvent	DMSO

Spectral properties

Excitation (nm)	385
Emission (nm)	529

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41105331
CAS	131474-38-9

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Page updated on December 13, 2024