D-Luciferin galactoside *CAS 131474-38-9*
D-Luciferin galactoside contains a beta-galactoside moiety attached at the 6-O-position, thus preventing it be recognized by the firefly luciferase enzyme. However, the galactose moiety is removed effectively by beta-galactosidase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin galactoside is a sensitive substrate for the chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors. When used as a dual substrate, beta-galactosidase (LacZ gene) and luciferase levels can be determined with high sensitivity. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light. It can also be used for in vivo bioluminescence imaging applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin galactoside *CAS 131474-38-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 226.009 µL | 1.13 mL | 2.26 mL | 11.3 mL | 22.601 mL |
5 mM | 45.202 µL | 226.009 µL | 452.018 µL | 2.26 mL | 4.52 mL |
10 mM | 22.601 µL | 113.005 µL | 226.009 µL | 1.13 mL | 2.26 mL |
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Product family
Name | Excitation (nm) | Emission (nm) |
D-Luciferin phosphate *CAS 145613-12-3* | 385 | 529 |
D-Luciferin acetate | 385 | 529 |
Citations
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References
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Authors: Masuda-Nishimura I, Fukuda S, Sano A, Kasai K, Tatsumi H.
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