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D-Luciferin methyl ester *CAS 73918-26-0*

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Physical properties
Molecular weight294.35
Spectral properties
Excitation (nm)385
Emission (nm)529
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
D-Luciferin methyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin methyl ester *CAS 73918-26-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM339.732 µL1.699 mL3.397 mL16.987 mL33.973 mL
5 mM67.946 µL339.732 µL679.463 µL3.397 mL6.795 mL
10 mM33.973 µL169.866 µL339.732 µL1.699 mL3.397 mL

Molarity calculator

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Spectral properties

Excitation (nm)385
Emission (nm)529

Product Family

NameExcitation (nm)Emission (nm)
D-Luciferin ethyl ester *CAS 135251-85-3*385529



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View all 113 references: Citation Explorer
Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants
Authors: Doyle TC, Nawotka KA, Purchio AF, Akin AR, Francis KP, Contag PR.
Journal: Microb Pathog (2006): 69
cDNA cloning, expression and homology modeling of a luciferase from the firefly Lampyroidea maculata
Authors: Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M.
Journal: J Biochem Mol Biol (2006): 578
Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor
Authors: Oba Y, Sato M, Inouye S.
Journal: Insect Mol Biol (2006): 293
Structural basis for the spectral difference in luciferase bioluminescence
Authors: Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H.
Journal: Nature (2006): 372
Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation
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Authors: Liu L, Hastings JW.
Journal: Proc Natl Acad Sci U S A. (2006)
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Authors: Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS.
Journal: Neurosurgery (2006): 365
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