D-Luciferin | AAT Bioquest

D-Luciferin phosphate

CAS 145613-12-3

D-Luciferin phosphate is a highly sensitive bioluminescent substrate for detecting alkaline phosphatases and protein phosphatases by luminometric detection. It is used in combination with luciferase/ATP system. D-Luciferin phosphate can be converted to D-Luciferin by phosphatases (such as alkaline phosphatases, acid phosphatases and protein phosphatases) that gives luminescence in the presence of luciferase and its cofactors. Our Amplite luciferase assay kit can be used for detecting D-luciferin release from D-Luciferin phosphate by phosphatases.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12512	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin phosphate *CAS 145613-12-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	234.604 µL	1.173 mL	2.346 mL	11.73 mL	23.46 mL
5 mM	46.921 µL	234.604 µL	469.208 µL	2.346 mL	4.692 mL
10 mM	23.46 µL	117.302 µL	234.604 µL	1.173 mL	2.346 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	426.25 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity

Authors:

Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan

Journal:

Journal of Extracellular Vesicles (2024): e12490

Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus

Authors:

Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco

Journal:

Proceedings of the National Academy of Sciences (2024): e2316646121

GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs

Authors:

Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,

Journal:

Plant Physiology (2023): kiad445

Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea

Authors:

Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,

Journal:

Bioorganic Chemistry (2023): 106625

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition

Authors:

Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan

Journal:

Oncogenesis (2022): 1--9

References

View all 22 references: Citation Explorer

Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI

Authors:

Zhao D, Richer E, Antich PP, Mason RP.

Journal:

Faseb J. (2008)

Mechanism of the stress-induced collapse of the Ran distribution

Authors:

Yasuda Y, Miyamoto Y, Saiwaki T, Yoneda Y.

Journal:

Exp Cell Res (2006): 512

Creatine phosphate administration preserves myocardial function in a model of off-pump coronary revascularization

Authors:

Woo YJ, Gr and TJ, Zentko S, Cohen JE, Hsu V, Atluri P, Berry MF, Taylor MD, Moise MA, Fisher O, Kolakowski S.

Journal:

J Cardiovasc Surg (Torino) (2005): 297

Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface

Authors:

Burrell HE, Wlodarski B, Foster BJ, Buckley KA, Sharpe GR, Quayle JM, Simpson AW, Gallagher JA.

Journal:

J Biol Chem (2005): 29667

Mechanically driven ATP synthesis by F1-ATPase

Authors:

Itoh H, Takahashi A, Adachi K, Noji H, Yasuda R, Yoshida M, Kinosita K.

Journal:

Nature (2004): 465

Physical properties

Molecular weight	426.25
Solvent	Water

Spectral properties

Excitation (nm)	385
Emission (nm)	529

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41105331
CAS	145613-12-3

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Page updated on December 13, 2024