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D-Luciferin phosphate *CAS 145613-12-3*

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Physical properties
Molecular weight426.25
Spectral properties
Excitation (nm)385
Emission (nm)529
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
D-Luciferin phosphate is a highly sensitive bioluminescent substrate for detecting alkaline phosphatases and protein phosphatases by luminometric detection. It is used in combination with luciferase/ATP system. D-Luciferin phosphate can be converted to D-Luciferin by phosphatases (such as alkaline phosphatases, acid phosphatases and protein phosphatases) that gives luminescence in the presence of luciferase and its cofactors. Our Amplite luciferase assay kit can be used for detecting D-luciferin release from D-Luciferin phosphate by phosphatases.

Example protocol


Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt.


Working solution should be prepared in water or DMSO.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin phosphate *CAS 145613-12-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM234.604 µL1.173 mL2.346 mL11.73 mL23.46 mL
5 mM46.921 µL234.604 µL469.208 µL2.346 mL4.692 mL
10 mM23.46 µL117.302 µL234.604 µL1.173 mL2.346 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


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Spectral properties

Excitation (nm)385
Emission (nm)529

Product Family

NameExcitation (nm)Emission (nm)
D-Luciferin galactoside *CAS 131474-38-9*385529
D-Luciferin acetate385529



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Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI
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Journal: Nature (2004): 465
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