D-Luciferin phosphate CAS 145613-12-3

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Physical properties

Molecular weight	426.25
Solvent	Water

Spectral properties

Excitation (nm)	385
Emission (nm)	529

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41105331

Overview SDS Protocol

CAS

145613-12-3

Molecular weight

426.25

Excitation (nm)

385

Emission (nm)

529

D-Luciferin phosphate is a highly sensitive bioluminescent substrate for detecting alkaline phosphatases and protein phosphatases by luminometric detection. It is used in combination with luciferase/ATP system. D-Luciferin phosphate can be converted to D-Luciferin by phosphatases (such as alkaline phosphatases, acid phosphatases and protein phosphatases) that gives luminescence in the presence of luciferase and its cofactors. Our Amplite luciferase assay kit can be used for detecting D-luciferin release from D-Luciferin phosphate by phosphatases.

Example protocol

AT A GLANCE

Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt.

PREPARATION OF WORKING SOLUTION

Working solution should be prepared in water or DMSO.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin phosphate *CAS 145613-12-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	234.604 µL	1.173 mL	2.346 mL	11.73 mL	23.46 mL
5 mM	46.921 µL	234.604 µL	469.208 µL	2.346 mL	4.692 mL
10 mM	23.46 µL	117.302 µL	234.604 µL	1.173 mL	2.346 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	385
Emission (nm)	529

Product Family

Name	Excitation (nm)	Emission (nm)
D-Luciferin galactoside CAS 131474-38-9	385	529
D-Luciferin acetate	385	529

Images

Figure 1. Chemical structure for D-Luciferin phosphate *CAS 145613-12-3*

Citations

View all 5 citations: Citation Explorer

Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121

GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445

Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9

Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

References

View all 22 references: Citation Explorer

Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI
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Creatine phosphate administration preserves myocardial function in a model of off-pump coronary revascularization
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Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface
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Journal: J Biol Chem (2005): 29667

Mechanically driven ATP synthesis by F1-ATPase
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Journal: Nature (2004): 465

Novel bioluminescent assay of alkaline phosphatase using adenosine-3'-phosphate-5'-phosphosulfate as substrate and the luciferin-luciferase reaction and its application
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Journal: Anal Biochem (2003): 206

Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26
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Journal: Cell Calcium (2003): 37

Mitochondrial membrane potential and intracellular ATP content after transient experimental ischemia in the cultured hippocampal neuron
Authors: Iijima T, Mishima T, Tohyama M, Akagawa K, Iwao Y.
Journal: Neurochem Int (2003): 263

Highly sensitive simultaneous bioluminescent measurement of acetate kinase and pyruvate phosphate dikinase activities using a firefly luciferase-luciferin reaction and its application to a tandem bioluminescent enzyme immunoassay
Authors: Ito K, Nakagawa K, Murakami S, Arakawa H, Maeda M.
Journal: Anal Sci (2003): 105

ATP released from astrocytes during swelling activates chloride channels
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Journal: J Neurophysiol (2003): 1870