D-Luciferin methyl ester *CAS 73918-26-0*

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Chemical structure for D-Luciferin methyl ester *CAS 73918-26-0*
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5 mg 12514 $195

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Ex/Em (nm)328/533
CAS #73918-26-0
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Category Cell Biology
Reporter Gene Enzymes
Related Hydrolytic Enzymes
Secondary Reagents
D-Luciferin methyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin methyl ester *CAS 73918-26-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
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