FastClick™ Biotin Alkyne
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 552.74 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ Biotin Azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 552.74 |
FastClick™ Biotin Alkyne contains both the CAG moiety of FastClick (for assisting click efficiency) and biotin (as the detection tag) for developing biotin-based probes. It readily reacts with an azido-containing biomolecule under extremely mild conditions. Biotin is one of the most commonly used tags that finds a variety of utilities in biological detections. Its conjugates are widely used in fluorescence imaging, fluorescence in situ hybridization (FISH) and nucleic acid detections in combination with the fluorescence-labeled streptavidin, avidin or NeutrAvidin® or other biotin-binding protein conjugates. AAT Bioquest offers the largest collection of biotin and streptavidin products for immunological detections. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ Biotin Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 180.917 µL | 904.584 µL | 1.809 mL | 9.046 mL | 18.092 mL |
5 mM | 36.183 µL | 180.917 µL | 361.834 µL | 1.809 mL | 3.618 mL |
10 mM | 18.092 µL | 90.458 µL | 180.917 µL | 904.584 µL | 1.809 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
FastClick™ Cy3 Alkyne | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
FastClick™ Cy5 Alkyne | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
FastClick™ Cy7 Alkyne | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
FastClick™ XFD350 Alkyne | 343 | 441 | 19000 | - | 0.25 | 0.19 |
FastClick™ XFD488 Alkyne | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
FastClick™ XFD555 Alkyne | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
FastClick™ XFD647 Alkyne | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
FastClick™ XFD750 Alkyne | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Images
Figure 1. The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
References
View all 16 references: Citation Explorer
Bioorthogonal Chemical Labeling Probes Targeting Sialic Acid Isomers for N-Glycan MALDI Imaging Mass Spectrometry of Tissues, Cells, and Biofluids.
Authors: Lu, Xiaowei and McDowell, Colin T and Blaschke, Calvin R K and Liu, Liping and Grimsley, Grace and Wisniewski, Luke and Gao, ChongFeng and Mehta, Anand S and Haab, Brian B and Angel, Peggi M and Drake, Richard R
Journal: Analytical chemistry (2023): 7475-7486
Authors: Lu, Xiaowei and McDowell, Colin T and Blaschke, Calvin R K and Liu, Liping and Grimsley, Grace and Wisniewski, Luke and Gao, ChongFeng and Mehta, Anand S and Haab, Brian B and Angel, Peggi M and Drake, Richard R
Journal: Analytical chemistry (2023): 7475-7486
Quantitative BONCAT Allows Identification of Newly Synthesized Proteins after Optic Nerve Injury.
Authors: Shah, Sahil H and Schiapparelli, Lucio M and Yokota, Satoshi and Ma, Yuanhui and Xia, Xin and Shankar, Sahana and Saturday, Sarah and Nahmou, Michael and Sun, Catalina and Yates, John and Cline, Hollis T and Goldberg, Jeffrey L
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (2022): 4042-4052
Authors: Shah, Sahil H and Schiapparelli, Lucio M and Yokota, Satoshi and Ma, Yuanhui and Xia, Xin and Shankar, Sahana and Saturday, Sarah and Nahmou, Michael and Sun, Catalina and Yates, John and Cline, Hollis T and Goldberg, Jeffrey L
Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (2022): 4042-4052
Tagging and Capture of Prenylated CaaX-Proteins from Plant Cell Cultures.
Authors: Ribeiro, Iliana and Ducos, Eric and Giglioli-Guivarc'h, Nathalie and Dutilleul, Christelle
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 241-248
Authors: Ribeiro, Iliana and Ducos, Eric and Giglioli-Guivarc'h, Nathalie and Dutilleul, Christelle
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 241-248
Dynamics of Non-Canonical Amino Acid-Labeled Intra- and Extracellular Proteins in the Developing Mouse.
Authors: Saleh, Aya M and Jacobson, Kathryn R and Kinzer-Ursem, Tamara L and Calve, Sarah
Journal: Cellular and molecular bioengineering (2019): 495-509
Authors: Saleh, Aya M and Jacobson, Kathryn R and Kinzer-Ursem, Tamara L and Calve, Sarah
Journal: Cellular and molecular bioengineering (2019): 495-509
Novel biotin linker with alkyne and amino groups for chemical labelling of a target protein of a bioactive small molecule.
Authors: Anabuki, Tomoaki and Tsukahara, Miu and Okamoto, Masanori and Matsuura, Hideyuki and Takahashi, Kosaku
Journal: Bioorganic & medicinal chemistry letters (2018): 783-786
Authors: Anabuki, Tomoaki and Tsukahara, Miu and Okamoto, Masanori and Matsuura, Hideyuki and Takahashi, Kosaku
Journal: Bioorganic & medicinal chemistry letters (2018): 783-786
Multiwalled carbon nanotubes for drug delivery: Efficiency related to length and incubation time.
Authors: Sciortino, Niccolò and Fedeli, Stefano and Paoli, Paolo and Brandi, Alberto and Chiarugi, Paola and Severi, Mirko and Cicchi, Stefano
Journal: International journal of pharmaceutics (2017): 69-72
Authors: Sciortino, Niccolò and Fedeli, Stefano and Paoli, Paolo and Brandi, Alberto and Chiarugi, Paola and Severi, Mirko and Cicchi, Stefano
Journal: International journal of pharmaceutics (2017): 69-72
Tandem photoaffinity labeling of a target protein using a linker with biotin, alkyne and benzophenone groups and a bioactive small molecule with an azide group.
Authors: Anabuki, Tomoaki and Tsukahara, Miu and Matsuura, Hideyuki and Takahashi, Kosaku
Journal: Bioscience, biotechnology, and biochemistry (2016): 432-9
Authors: Anabuki, Tomoaki and Tsukahara, Miu and Matsuura, Hideyuki and Takahashi, Kosaku
Journal: Bioscience, biotechnology, and biochemistry (2016): 432-9
ASG2 is a farnesylated DWD protein that acts as ABA negative regulator in Arabidopsis.
Authors: Dutilleul, Christelle and Ribeiro, Iliana and Blanc, Nathalie and Nezames, Cynthia D and Deng, Xing Wang and Zglobicki, Piotr and Palacio Barrera, Ana María and Atehortùa, Lucia and Courtois, Martine and Labas, Valérie and Giglioli-Guivarc'h, Nathalie and Ducos, Eric
Journal: Plant, cell & environment (2016): 185-98
Authors: Dutilleul, Christelle and Ribeiro, Iliana and Blanc, Nathalie and Nezames, Cynthia D and Deng, Xing Wang and Zglobicki, Piotr and Palacio Barrera, Ana María and Atehortùa, Lucia and Courtois, Martine and Labas, Valérie and Giglioli-Guivarc'h, Nathalie and Ducos, Eric
Journal: Plant, cell & environment (2016): 185-98
Proteome-Wide Profiling of Targets of Cysteine reactive Small Molecules by Using Ethynyl Benziodoxolone Reagents.
Authors: Abegg, Daniel and Frei, Reto and Cerato, Luca and Prasad Hari, Durga and Wang, Chao and Waser, Jerome and Adibekian, Alexander
Journal: Angewandte Chemie (International ed. in English) (2015): 10852-7
Authors: Abegg, Daniel and Frei, Reto and Cerato, Luca and Prasad Hari, Durga and Wang, Chao and Waser, Jerome and Adibekian, Alexander
Journal: Angewandte Chemie (International ed. in English) (2015): 10852-7
Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices.
Authors: Feidenhans'l, Nikolaj A and Lafleur, Josiane P and Jensen, Thomas G and Kutter, Jörg P
Journal: Electrophoresis (2014): 282-8
Authors: Feidenhans'l, Nikolaj A and Lafleur, Josiane P and Jensen, Thomas G and Kutter, Jörg P
Journal: Electrophoresis (2014): 282-8