iFluor® 440-dUTP *1 mM in TE Buffer (pH 7.5)*

iFluor® 440-dUTP

1 mM in TE Buffer (pH 7.5)

iFluor® 440-dUTP is a fluorescent nucleotide analog for enzymatic incorporation into DNA, enabling the preparation of labeled probes for nucleic acid detection and hybridization applications.

Enzymatic DNA Labeling: Enables incorporation of fluorescent nucleotides into DNA during enzymatic synthesis.
Spectral Properties: Excitation at 434 nm and emission at 480 nm.
Fluorescence Performance: Water-soluble dye with manufacturer-reported enhanced brightness compared with DEAC conjugates.
Applications: Suitable for FISH probe preparation and microarray analysis.

Chemical structure for iFluor® 440-dUTP *1 mM in TE Buffer (pH 7.5)*.

Datasheet

Catalog	Size	Price	Quantity
17029	25 nmoles	Price
17029-100NM	100 nmoles	Price

Overview

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. DEAC-dUTP conjugate (#17011) is widely used as a blue fluorescence color with SpectrumAqua™ filter set (SpectrumAqua™ is the trademark of Vysis) since DEAC and SpectrumAqua have remarkably similar spectral properties. However, the extremely high hydrophobicity of DECA fluorophore make the DEAC conjugates easily adsorbed on plastic surfaces, resulting in high assay errors. iFluor® 440 dyes have excellent water solubility and are designed to be a superior replacement for DEAC dye. iFluor® 440-dUTP conjugate is a superior replacement for DEAC-dUTP conjugate. iFluor® 440 dye conjugates are significantly brighter than the corresponding bioconjugates of DEAC or other spectrally similar dyes (such as SpectrumAqua). iFluor® 440 fluorophore has its spectra well matched with the filter set of SpectrumAqua (SpectrumAqua™ is the trademark of Vysis).

References

View all 8 references: Citation Explorer

MECOM (EVI1) Rearrangements: A Review and Case Report of Two MDS Patients with Complex 3q Inversion/Deletions.

Authors:

Lawce, Helen and Szabo, Elina and Torimaru, Yumi and Davis, Craig and Osterberg, Karin and Olson, Susan and Moore, Steve

Journal:

Journal of the Association of Genetic Technologists (2017): 9-14

Localization of a female-specific marker on the chromosomes of the brown seaweed Saccharina japonica using fluorescence in situ hybridization.

Authors:

Liu, Yu and Bi, YanHui and Gu, JunGang and Li, LiHua and Zhou, ZhiGang

Journal:

PloS one (2012): e48784

Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice.

Authors:

Rojo, Marcial García and Bueno, Gloria and Slodkowska, Janina

Journal:

Folia histochemica et cytobiologica (2009): 349-54

Application of tri-colour, dual fusion fluorescence in situ hybridization (FISH) system for the characterization of BCR-ABL1 fusion in chronic myelogenous leukaemia (CML) and residual disease monitoring.

Authors:

Siu, Lisa Lp and Ma, Edmond Sk and Wong, Wai Shan and Chan, Man Hong and Wong, Kit Fai

Journal:

BMC blood disorders (2009): 4

Bladder cancer detection using FISH (UroVysion assay).

Authors:

Halling, Kevin C and Kipp, Benjamin R

Journal:

Advances in anatomic pathology (2008): 279-86

Physical properties

Molecular weight	994.73
Solvent	Water

Spectral properties

Absorbance (nm)	430
Correction factor (260 nm)	0.352
Correction factor (280 nm)	0.229
Extinction coefficient (cm ^-1 M ^-1)	40000 ¹
Excitation (nm)	434
Emission (nm)	480
Quantum yield	0.67 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 24, 2026