mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | ~1000 |
| Solvent | Water |
Spectral properties
| Absorbance (nm) | 406 |
| Correction Factor (260 nm) | 0.338 |
| Correction Factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 350001 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.811 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
| Overview |  SDSProtocol |
See also: Fluorescent in situ hybridization (FISH), mFluor™ Dyes and Kits, RNA Purification & Analysis
Molecular weight ~1000 | Absorbance (nm) 406 | Correction Factor (260 nm) 0.338 | Correction Factor (280 nm) 0.078 | Extinction coefficient (cm -1 M -1) 350001 | Excitation (nm) 406 | Emission (nm) 445 | Quantum yield 0.811 |
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This mFluor™ 450-dUTP conjugate can be used as a blue fluorescence color with Pacific Blue® filter set. (Pacific Blue® is the trademark of Invitrogen).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 406 |
| Correction Factor (260 nm) | 0.338 |
| Correction Factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 350001 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.811 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 450 SE | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
| mFluor™ Violet 450 acid | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
| mFluor™ Violet 450 Azide | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
| mFluor™ Violet 450 maleimide | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
Images
Figure 1. Chemical structure for mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*
References
View all 9 references: Citation Explorer
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Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
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Journal: Biosens Bioelectron (2004): 685
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
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Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
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Journal: J Cell Biochem (1991): 166
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953