logo
AAT Bioquest

mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight~1000
SolventWater
Spectral properties
Absorbance (nm)406
Correction Factor (260 nm)0.338
Correction Factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)350001
Excitation (nm)406
Emission (nm)445
Quantum yield0.811
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Related products
mFluor™ Violet 510 SE
mFluor™ Violet 540 SE
mFluor™ Blue 570 SE
mFluor™ Green 620 SE
mFluor™ Yellow 630 SE
mFluor™ Red 700 SE
mFluor™ Red 780 SE
mFluor™ Red 780 amine
mFluor™ Violet 450-VAD-FMK
mFluor™ 510-VAD-FMK
mFluor™ Violet 450-streptavidin conjugate
mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Red 780-streptavidin conjugate
mFluor™ Violet 510 acid
mFluor™ Violet 540 acid
mFluor™ Blue 570 acid
mFluor™ Green 620 acid
mFluor™ Yellow 630 acid
mFluor™ Red 700 acid
mFluor™ Violet 500 SE
mFluor™ UV375 SE
mFluor™ Red 780 acid
mFluor™ UV460 SE
mFluor™ Blue 630 SE
mFluor™ Blue 660 SE
mFluor™ Violet 610 SE
mFluor™ Green 630 SE
mFluor™ Blue 580 SE
mFluor™ Blue 590 SE
mFluor™ Blue 620 SE
mFluor™ Red 780 Maleimide
mFluor™ Violet 550 SE
mFluor™ Violet 505 SE
mFluor™ Violet 590 SE
mFluor™ Violet 545 SE
mFluor™ UV420 SE
mFluor™ UV455 SE
mFluor™ UV520 SE
mFluor™ UV540 SE
mFluor™ UV610 SE
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate
mFluor™ Violet 530 SE
mFluor™ Violet 530 maleimide
mFluor™ Violet 480 SE
mFluor™ Blue 660 tyramide
mFluor™ Violet 450-PEG4-Biotin Conjugate
mFluor™ Violet 450-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate
mFluor™ Blue 585 SE
mFluor™ Blue 583 SE
mFluor™ Blue 580 Styramide
mFluor™ Blue 630 Styramide
mFluor™ Blue 660 Styramide
mFluor™ Green 620 Styramide
mFluor™ Red 780 Styramide
mFluor™ Violet 540 Styramide
mFluor™ Violet 545 Styramide
mFluor™ Violet 610 Styramide
mFluor™ UV455-streptavidin conjugate
mFluor™ Violet 545-streptavidin conjugate
mFluor™ Violet 550-streptavidin conjugate
mFluor™ Violet 590-streptavidin conjugate
mFluor™ Violet 610-streptavidin conjugate
mFluor™ UV 375 goat anti-mouse IgG (H+L)
mFluor™ UV 375 goat anti-rabbit IgG (H+L)
mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-mouse IgG (H+L)
mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-rabbit IgG (H+L)
mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-rabbit IgG (H+L)
mFluor™ Red 780 goat anti-mouse IgG (H+L) *Cross-Absorbed*
mFluor™ Red 780 goat anti-mouse IgG (H+L)
mFluor™ Blue 585 Anti-human CD4 Antibody *SK3*
mFluor™ UV420-streptavidin conjugate
Show More (77)

OverviewpdfSDSpdfProtocol


Molecular weight
~1000
Absorbance (nm)
406
Correction Factor (260 nm)
0.338
Correction Factor (280 nm)
0.078
Extinction coefficient (cm -1 M -1)
350001
Excitation (nm)
406
Emission (nm)
445
Quantum yield
0.811
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This mFluor™ 450-dUTP conjugate can be used as a blue fluorescence color with Pacific Blue® filter set. (Pacific Blue® is the trademark of Invitrogen).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)406
Correction Factor (260 nm)0.338
Correction Factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)350001
Excitation (nm)406
Emission (nm)445
Quantum yield0.811

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450 SE4064453500010.8110.3380.078
mFluor™ Violet 450 acid4064453500010.8110.3380.078
mFluor™ Violet 450 Azide4064453500010.8110.3380.078
mFluor™ Violet 450 maleimide4064453500010.8110.3380.078

Images


References


View all 9 references: Citation Explorer
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953