ProLite™ His-Tag Protein Gel Staining Kit Green Fluorescence

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Platform

Gel Imager

Excitation	Blue laser
Emission	Long path green filter (SYBR filter)

Example protocol

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

ProLite™ His-Tag Protein Gel Stain stock solution

Prepare a ProLite™ His-Tag Protein Gel Stain stock solution by adding 60 µL of DMSO to a vial of ProLite™ His-Tag Protein Gel Stain. Note: 1 vial of stock solution is suitable for two gels. Store any unused stock solution at -20 °C.

PREPARATION OF WORKING SOLUTION

ProLite™ His Tag Protein Gel Stain working solution

Prepare a ProLite™ His-Tag Protein Gel Stain working solution by adding 30 µL of ProLite™ His-Tag Protein Gel Stain stock solution to 30 mL of PBS. Note: Make enough working solution that the gel is completely immersed in the working solution. Do not reuse the working solution.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol should be used as a guideline.

Post-staining protocol

Run gels based on your standard protocol.
Place the gel in a suitable container, and fix the gel in a fixing solution for 60 minutes. Note: 40% ethanol + 10% acetic acid can be used as a fixing solution.
Wash the gel twice with ultra-pure water.
Incubate the gel in the ProLite™ His-Tag Protein Gel Stain working solution for 60 minutes. Be sure the gel is immersed in the working solution.
Remove the working solution and wash the gel twice with PBS.
Proceed to imaging the gel immediately.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Images

Figure 1. Two-fold dilution series of His-tagged annexin V were separated on a NuPAGE® 4–12% Bis-Tris gel and stained with the ProLite™ His-Tag Protein Gel Staining Kit according to standard protocols. Lane 1: His-tagged protein ladder, Lane 2 to 5: two-fold dilution of His-tagged annexin V.

References

View all 43 references: Citation Explorer

Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair.
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223

A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654

The surface syndecan protein from Macrobrachium rosenbergii could function as mediator in bacterial infections.
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68

[Cloning and expression of SmDXS2 gene in Swertia mussotii].
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941

Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System.
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379

Biochemical and Functional Characterization of Mouse Mammary Tumor Virus Full-Length Pr77Gag Expressed in Prokaryotic and Eukaryotic Cells.
Authors: Chameettachal, Akhil and Pillai, Vineeta Narayana and Ali, Lizna Mohamed and Pitchai, Fathima Nuzra Nagoor and Ardah, Mustafa Taleb and Mustafa, Farah and Marquet, Roland and Rizvi, Tahir Aziz
Journal: Viruses (2018)

Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs.
Authors: Huang, Jingwei and Xiong, Kang and Zhang, Houshuang and Zhao, Yanzhen and Cao, Jie and Gong, Haiyan and Zhou, Yongzhi and Zhou, Jinlin
Journal: Parasites & vectors (2018): 38

Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles.
Authors: Torres-González, Lisa and Díaz-Ayala, Ramonita and Vega-Olivencia, Carmen A and López-Garriga, Juan
Journal: Sensors (Basel, Switzerland) (2018)

Facile fabrication of nickel immobilized on magnetic nanoparticles as an efficient affinity adsorbent for purification of his-tagged protein.
Authors: Rashid, Zahra and Naeimi, Hossein and Zarnani, Amir-Hassan and Mohammadi, Fereshteh and Ghahremanzadeh, Ramin
Journal: Materials science & engineering. C, Materials for biological applications (2017): 670-676

Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates.
Authors: Benjamin, Christopher J and Wright, Kyle J and Hyun, Seok-Hee and Krynski, Kyle and Yu, Guimei and Bajaj, Ruchika and Guo, Fei and Stauffacher, Cynthia V and Jiang, Wen and Thompson, David H
Journal: Langmuir : the ACS journal of surfaces and colloids (2016): 551-9