Name: ReadiUse™ Preactivated PE-iFluor® 594 Tandem
Brand: AAT Bioquest
SKU: 2584
Price: 415 USD
Availability: InStock

ReadiUse™ Preactivated PE-iFluor® 594 Tandem

ReadiUse™ Preactivated PE-iFluor® 594 Tandem is a ready-to-conjugate fluorescent tandem dye for labeling antibodies and other proteins for multicolor flow cytometry.

Fluorescence Detection: Excitation at 565 nm and emission at 606 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated dye and Buccutite™ MTA reagent.
Improved Performance: Brighter fluorescence and enhanced FRET efficiency compared to PE-Texas Red.

Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 594 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
2584	1 mg	Price

Overview

PE-Texas Red is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 600 nm. AAT Bioquest offers this preactivated PE-iFluor® 594™ as a superior replacement to the popular PE-Texas Red tandem. Compared to PE-Texas Red tandem, PE-iFluor® 594 is brighter with better FRET efficiency. It is used to facilitate the PE-Texas Red tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-iFluor® 594 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based PE-Texas Red tandem conjugation chemistry with better flow cytometry peformance. In addition, our preactivated PE-iFluor® 594 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	606

Usage and storage

Intended use

Research Use Only (RUO)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

A: ReadiUse™ Preactivated PE-iFluor® 594 Tandem	1 vial (1 mg)
B: Buccutite™ MTA	1 vial (100 μg)
C: Spin Desalting Column	Not Included