Name: Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein*
Brand: AAT Bioquest
SKU: 2248
Price: 232 USD
Availability: InStock

Tide Fluor™ 2, succinimidyl ester

TF2 SE; Superior replacement for fluorescein

Tide Fluor™ 2 (TF2) family has the spectral properties similar to those of fluoresceins and Alexa Fluor® 488 (Alexa Fluor® is the trademark of Invitrogen). Compared to fluoresceins TF2 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluoresceins. TF2-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with FITC or FAM. In pairing with our Tide Quencher™ 2 (TQ2), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. In addition, TF2 has spectral properties, pH dependence and photostability similar to Alexa Fluor® 488, making them excellent replacement for the expensive Alexa Fluor® 488.

Photostability analysis of Tide Fluor™ 2 and FITC.

Catalog	Size	Price	Quantity
2248	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 2, succinimidyl ester [TF2 SE]*Superior replacement for fluorescein* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	171.063 µL	855.315 µL	1.711 mL	8.553 mL	17.106 mL
5 mM	34.213 µL	171.063 µL	342.126 µL	1.711 mL	3.421 mL
10 mM	17.106 µL	85.531 µL	171.063 µL	855.315 µL	1.711 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	584.58 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

Intraocular Delivery of a Collagen Mimetic Peptide Repairs Retinal Ganglion Cell Axons in Chronic and Acute Injury Models

Authors:

Ribeiro, Marcio and McGrady, Nolan R and Baratta, Robert O and Del Buono, Brian J and Schlumpf, Eric and Calkins, David J

Journal:

International Journal of Molecular Sciences (2022): 2911

Analysis of critical quality attributes in monoclonal antibodies for upstream process development

Authors:

Oshinbolu, Sheun

Cloning and characteristic of MMP1 gene from Hyriopsis cumingii and collagen hydrolytic activity of its recombinant protein

Authors:

Hu, Baoqing and Xiao, Jun and Yi, Peipei and Hu, Chenxi and Zhu, Mingxing and Yin, Shuyuan and Wen, Chungen and Wu, Jielian

Journal:

Gene (2019)

The dynamic nature of transglutaminases

Authors:

Hjorth-Jensen, Samuel

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation

Authors:

Perlenfein, Tyler J and Murphy, Regina M

Journal:

Journal of Biological Chemistry (2017): jbc--M117

References

View all 25 references: Citation Explorer

Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers

Authors:

Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.

Journal:

J Med Chem (2009): 358

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe

Authors:

Andreoni A, Bondani M, Nardo L.

Journal:

Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer

Authors:

Ogawa M, Kosaka N, Choyke PL, Kobayashi H.

Journal:

Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Authors:

Kim GI, Kim KW, Oh MK, Sung YM.

Journal:

Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening

Authors:

Yu X, Sainz B, Jr., Uprichard SL.

Journal:

Antimicrob Agents Chemother (2009): 4311

Physical properties

Molecular weight	584.58
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	75000
Excitation (nm)	503
Emission (nm)	525
Quantum yield	0.9 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 25, 2025