Is phalloidin cell permeable?

No phalloidin is not cell permeable and therefore should not be used on living cells. Fluorescently labeled phalloidins, such as Phalloidin-iFluor® 488 Conjugate, can only be used to stain F-actin in formaldehyde-fixed and permeabilized tissue sections, cell culture and cell-free experiments.

For more information on phalloidin conjugates for f-actin detection, see 'Additional Resources' below.