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What is phalloidin staining used for?

Posted October 24, 2019


Answer

Phalloidin is a highly selective peptide that is used for staining filamentous actin (F-actin) in formaldehyde-fixed and permeabilized tissue sections, cell-cultures and cell-free experiments. Phalloidin is commonly labeled with organic fluorophores such as iFluor® dyes to facilitate visualization.

It is important to note that fluorescently-labeled phalloidins are not cell permeable and therefore cannot be used on living cells.

Additional resources

Cell Structures and Organelles: Actin