AAT Bioquest

What are the 3 binding sites for tRNA in the ribosome?

Posted June 22, 2020


The 3 binding sites for tRNA are called aminoacyl site (abbreviated A), the peptidyl site (abbreviated P) and the exit site (abbreviated E), which are oriented 5’ to 3’ E-P-A with respect to the mRNA.

  • The A site binds to the incoming aminoacyl tRNA, which carries the new amino acid to be added to the polypeptide chain.
  • The P site holds the tRNA with the growing polypeptide chain.
  • The E site serves as a threshold. It holds the tRNA without its amino acid, which is then released by ribosome.
Additional resources

Helixyte™ Green *10,000X Aqueous PCR Solution*

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Perdew, G. H., Heuvel, J. P. V., & Peters, J. M. (2008). Regulation of gene expression. Springer Science & Business Media.

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.

Transfectamine™ mRNA Transfection Reagent