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What is the difference between PAGE and SDS-PAGE?

Posted June 1, 2020


The major difference between native PAGE and SDS-PAGE is that in native PAGE, the protein migration rate is dependent on both the mass and structure, whereas in SDS-PAGE, the migration rate is determined only by protein’s mass.

In native PAGE, protein samples are prepared in a non-denaturing and non-reducing buffer. Therefore, in addition to the size of protein, the secondary, tertiary as well as quaternary structure all has an impact on the migration.

In SDS-PAGE, an excessive amount of SDS is added to coat proteins in negative charge, which covers all the intrinsic charges of protein. A similar charge-to-mass ratio is obtained for all proteins, and the electrophoretic mobility in SDS-PAGE depends almost solely on the size of protein.

Additional resources

Antibody development services

Gelite™ Green Nucleic Acid Gel Staining Kit

Sambrook, J., & Russell, D. W. (2006). SDS-polyacrylamide gel electrophoresis of proteins. CSH Protoc, 2006(4), pdb-prot4540.

SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe