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How do I label the cell membrane?

Posted November 19, 2022


Answer

The cell membrane can be labeled using Lipophilic Tracers, Fluorescently Labeled Lectins, and Fluorescent Probes. 

  • Lipophilic Tracers used to label the cell membrane include dialkylcarbocyanines DiO, DiI, DiA, DiD, DiR and DiS
  • Fluorescent Wheat Germ agglutinin (WGA) is a Fluorescently Labeled Lectin used extensively to label the cell membrane. 
  • Fluorescent probes of FM4-64, LRB-PE, DiIC12, DiIC18, DiD, BD-SM and Laurdan are most widely used to label cell membrane.
Additional resources

Fluorescent labelling of cell membranes and cytoplasmic proteins in living cells

Cell Signaling

Membrane Potential and Channels

RH 237 [N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt]