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How do I label the cell membrane?
Posted November 19, 2022
Cell SignalingCellular ProcessesMembrane Potential and ChannelsPhysiological ProbesPlasma Membrane
Answer

The cell membrane can be labeled using Lipophilic Tracers, Fluorescently Labeled Lectins, and Fluorescent Probes. 

  • Lipophilic Tracers used to label the cell membrane include dialkylcarbocyanines DiO, DiI, DiA, DiD, DiR and DiS. 
  • Fluorescent Wheat Germ agglutinin (WGA) is a Fluorescently Labeled Lectin used extensively to label the cell membrane. 
  • Fluorescent probes of FM4-64, LRB-PE, DiIC12, DiIC18, DiD, BD-SM and Laurdan are most widely used to label cell membrane.
Additional resources
Fluorescent labelling of cell membranes and cytoplasmic proteins in living cellsCell SignalingMembrane Potential and ChannelsRH 237 [N-(4-Sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, inner salt]

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