AAT Bioquest

What are the advantages of digital PCR (dPCR)?

Posted November 11, 2023


Digital PCR (dPCR) is a refinement of PCR where DNA is subject to PCR for amplification of the template, then the sample undergoes fluorescent detection and sequence-specific alleles can be directly counted.  As the third generation of the technique, dPCR has many benefits over its predecessor, RT-qPCR.

  • dPCR is not as vulnerable to contamination as fluorescence detection is a simplified process.
  • dPCR is also capable of providing absolute, versus relative, quantification, and can be used even with low copy number genes.
  • dPCR may be experimentally faster than RT-qPCR since it does not require a standard curve calibration, that also may require expensive reagents.
  • dPCR also offers increased resistance to PCR inhibitors over other techniques.
  • dPCR is independent of amplification efficiency, and results are extremely repeatable.
  • dPCR offers a highly sensitive quantification technique where only a very small sample is needed for starting material. 
Additional resources

Principles of digital PCR and its applications in current obstetrical and gynecological diseases

EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*

DABCYL C2 maleimide

Digital PCR

Tide Quencher™ 1 azide [TQ1 azide]