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Calcium Indicators
AAT Bioquest offers the largest selection of calcium indicators, with options for different modalities, binding affinities, and emission colors to optimally fit your experimental needs.
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Binding Affinities
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Binding Affinity (Kd)
Emission Color
No Probenecid Required
Excitation Wavelength
200 nm
900 nm
Kits
No Wash
Emission Wavelength
200 nm
900 nm
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Recommended
Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM]
Catalog Number: 20501, 20502
Modality
Single-wavelength
Excitation/Emission (nm)
498/517
Kd (nM)
190
Cal Green™ 1, hexapotassium salt
Catalog Number: 20500
Modality
Single-wavelength
Excitation/Emission (nm)
498/517
Kd (nM)
190
Fluo-2, AM
Catalog Number: 20494, 20495
Modality
Single-wavelength
Excitation/Emission (nm)
-
Kd (nM)
232
Fluo-2, potassium salt
Catalog Number: 20493
Modality
Single-wavelength
Excitation/Emission (nm)
-
Kd (nM)
232
Fluo-8H™, AM
Catalog Number: 21090, 21091
Modality
Single-wavelength
Excitation/Emission (nm)
495/516
Kd (nM)
232
Fluo-8H™, sodium salt
Catalog Number: 21095
Modality
Single-wavelength
Excitation/Emission (nm)
495/516
Kd (nM)
232
Fura-10™, AM
Catalog Number: 21114, 21115
Modality
Ratiometric
Excitation/Emission (nm)
354/524
Kd (nM)
260
Fura-10™, potassium salt
Catalog Number: 21110, 21111
Modality
Ratiometric
Excitation/Emission (nm)
354/524
Kd (nM)
260
Fura-2, AM CAS 108964-32-5
Catalog Number: 21020, 21022
Modality
Ratiometric
Excitation/Emission (nm)
336/505
Kd (nM)
145
Learn More
AAT Bioquest offers the largest portfolio of calcium indicators, with over one hundred unique reagents, cited in tens of thousands of publications. The table below offers a few example applications. To learn more about how to select the optimal indicator for your experiment, please see our Calcium Indicators catalog.
| What you're studying | What you're measuring | AAT Bioquest products to consider |
|---|---|---|
| Neuronal activity | Spontaneous activity, stimulus responses, and neuronal calcium transients | Cal-520® AM; Cal-590™ AM when combining calcium imaging with GFP. |
| Cardiac calcium handling | Beat-associated transients and changes in calcium release and recovery | Cal-520® AM or Calbryte™ 520 AM; evaluate signal kinetics and saturation in your model. |
| Immune-cell activation | Calcium flux after receptor stimulation in defined cell populations | Calbryte™ 520 AM for green-channel flow cytometry; Calbryte™ 630 AM for a compatible red-laser configuration. |
| Drug screening and receptor/channel assays | Compound-induced calcium responses and concentration–response curves | Screen Quest™ Calbryte™ 520 or Screen Quest™ Fluo-8® assays for streamlined, no-wash screening. |
| Calcium-store release and entry | Cytosolic calcium changes following store depletion or extracellular calcium addition | Fura-8™ AM for ratiometric measurements; Calbryte™ 520 AM for relative fluorescence changes. |
Resources & Tools
Calcium Indicators
Learn about the different factors when selecting an optimal calcium indicator
View Catalog
Cal-520® and Other Cal™ Indicators
Explore our Cal-520® and Other Cal™ Indicators digital catalog
View Catalog