iFluor® 405 azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	660.64
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.48
Correction Factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Molecular weight

660.64

Correction Factor (260 nm)

0.48

Correction Factor (280 nm)

0.77

Extinction coefficient (cm ^-1 M ^-1)

37000¹

Excitation (nm)

403

Emission (nm)

427

Quantum yield

0.91¹

iFluor® 405 azide is reasonably stable and shows good reactivity and selectivity with the alkyne group in click chemistry. iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 405 dyes have fluorescence excitation and emission maxima of ~403 nm and ~427 nm respectively. The iFluor® 405 family has spectral properties essentially identical to those of Alexa Fluor® 405 (Alexa Fluor® 405 is the trademark of ThermoFisher). Its fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a great alternative to Alexa Fluor® 405.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 405 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	151.368 µL	756.842 µL	1.514 mL	7.568 mL	15.137 mL
5 mM	30.274 µL	151.368 µL	302.737 µL	1.514 mL	3.027 mL
10 mM	15.137 µL	75.684 µL	151.368 µL	756.842 µL	1.514 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.48
Correction Factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 647 azide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 488 azide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 azide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 405 Styramide	403	427	37000¹	0.91¹	0.48	0.77
iFluor™ 790 Azide	787	812	250000¹	0.13¹	0.1	0.09

Images

Figure 1. iFluor® 405 azide is reasonably stable and shows good reactivity and selectivity with alkyne group in click chemistry. iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).

Citations

View all 1 citations: Citation Explorer

PF-D-Trimer, a protective SARS-CoV-2 subunit vaccine: immunogenicity and application
Authors: Zhang, Zhihao and Zhou, Jinhu and Ni, Peng and Hu, Bing and Jolicoeur, Normand and Deng, Shuang and Xiao, Qian and He, Qian and Li, Gai and Xia, Yan and others,
Journal: npj Vaccines (2023): 38

References

View all 5 references: Citation Explorer

Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845

Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74

Identification of novel cell-impermeant fluorescent substrates for testing the function and drug interaction of Organic Anion-Transporting Polypeptides, OATP1B1/1B3 and 2B1.
Authors: Patik, Izabel and Székely, Virág and Német, Orsolya and Szepesi, Áron and Kucsma, Nóra and Várady, György and Szakács, Gergely and Bakos, Éva and Özvegy-Laczka, Csilla
Journal: Scientific reports (2018): 2630

Energy transfer between a biological labelling dye and gold nanorods.
Authors: Racknor, Chris and Singh, Mahi R and Zhang, Yinan and Birch, David J S and Chen, Yu
Journal: Methods and applications in fluorescence (2013): 015002

Violet laser diodes in flow cytometry: an update.
Authors: Telford, William and Kapoor, Veena and Jackson, James and Burgess, Walter and Buller, Gayle and Hawley, Teresa and Hawley, Robert
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2006): 1153-60