iFluor® 540 maleimide

Ordering information

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	777.82
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 540 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	128.564 µL	642.822 µL	1.286 mL	6.428 mL	12.856 mL
5 mM	25.713 µL	128.564 µL	257.129 µL	1.286 mL	2.571 mL
10 mM	12.856 µL	64.282 µL	128.564 µL	642.822 µL	1.286 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
iFluor® 720 maleimide	716	740	240000¹	0.14¹	0.15	0.13
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References

View all 8 references: Citation Explorer

A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility.
Authors: Shrivastava, Abhishek and Berg, Howard C
Journal: Science advances (2020): eaay6616

Protein Environment and DNA Orientation Affect Protein-Induced Cy3 Fluorescence Enhancement.
Authors: Nguyen, Binh and Ciuba, Monika A and Kozlov, Alexander G and Levitus, Marcia and Lohman, Timothy M
Journal: Biophysical journal (2019): 66-73

Toward a solid-phase nucleic acid hybridization assay within microfluidic channels using immobilized quantum dots as donors in fluorescence resonance energy transfer.
Authors: Chen, Lu and Algar, W Russ and Tavares, Anthony J and Krull, Ulrich J
Journal: Analytical and bioanalytical chemistry (2011): 133-41

Electrical readout of protein microarrays on regular glass slides.
Authors: Bonilla, Diana and Mallén, Maria and de la Rica, Roberto and Fernández-Sánchez, César and Baldi, Antonio
Journal: Analytical chemistry (2011): 1726-31

Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer.
Authors: Menacher, Florian and Rubner, Moritz and Berndl, Sina and Wagenknecht, Hans-Achim
Journal: The Journal of organic chemistry (2008): 4263-6

Probing orientations of single fluorescent labels on a peptide reversibly binding to the human delta-opioid receptor.
Authors: Tokimoto, Takahira and Bethea, Tomika R C and Zhou, Min and Ghosh, Indraneel and Wirth, Mary J
Journal: Applied spectroscopy (2007): 130-7

Laser scanning and electron microscopic evidence for rapid and specific in vivo labelling of cholinergic neurons in the rat basal forebrain with fluorochromated antibodies.
Authors: Kacza, J and Grosche, J and Seeger, J and Brauer, K and Brückner, G and Härtig, W
Journal: Brain research (2000): 232-8

Stimulated release of fluorescently labeled IgE fragments that efficiently accumulate in secretory granules after endocytosis in RBL-2H3 mast cells.
Authors: Xu, K and Williams, R M and Holowka, D and Baird, B
Journal: Journal of cell science (1998): 2385-96