iFluor® 790 Alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1316.35 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.1 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 787 |
Emission (nm) | 812 |
Quantum yield | 0.131 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 1316.35 | Correction Factor (260 nm) 0.1 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 787 | Emission (nm) 812 | Quantum yield 0.131 |
iFluor® 790 Alkyne readily reacts with biomolecules that contain an azido group. It can be conveniently used to develop NIR imaging probers. Our iFluor® 790 dye is designed to label proteins and other biomolecules with near infrared fluorescence. Conjugates prepared with iFluor® 790 have the excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7 or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is extremely useful for small animal in-vivo imaging applications or for other imaging applications that require NIR detections such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system. In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments are aiming to achieve high-resolution, multimodality and lifetime-based in vivo fluorescence imaging.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of iFluor® 790 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 75.968 µL | 379.838 µL | 759.676 µL | 3.798 mL | 7.597 mL |
5 mM | 15.194 µL | 75.968 µL | 151.935 µL | 759.676 µL | 1.519 mL |
10 mM | 7.597 µL | 37.984 µL | 75.968 µL | 379.838 µL | 759.676 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.1 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 787 |
Emission (nm) | 812 |
Quantum yield | 0.131 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 647 alkyne | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 555 alkyne | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 488 alkyne | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide* | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
Images
Figure 1. iFluor® 790 Alkyne readily reacts with biomolecules that contain an azido group. It can be conveniently used to develop NIR imaging probers. Our iFluor™ 790 dye is designed to label proteins and other biomolecules with near infrared fluorescence. Conjugates prepared with iFluor™ 790 have the excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima.
References
View all 50 references: Citation Explorer
NIRDye 812: A molecular platform tailored for multimodal bioimaging applications of targeted fluorescence- and photoacoustic-guided surgery.
Authors: Hettie, Kenneth S and Chin, Frederick T
Journal: Journal of photochemistry and photobiology. B, Biology (2023): 112683
Authors: Hettie, Kenneth S and Chin, Frederick T
Journal: Journal of photochemistry and photobiology. B, Biology (2023): 112683
Preclinical Identification Of Tumor-Draining Lymph Nodes Using a Multimodal Non-invasive In vivo Imaging Approach.
Authors: Knopf, Philipp and Stowbur, Dimitri and Hoffmann, Sabrina H L and Fransen, Marieke F and Schwenck, Johannes and Pichler, Bernd J and Kneilling, Manfred
Journal: Molecular imaging and biology (2023): 606-618
Authors: Knopf, Philipp and Stowbur, Dimitri and Hoffmann, Sabrina H L and Fransen, Marieke F and Schwenck, Johannes and Pichler, Bernd J and Kneilling, Manfred
Journal: Molecular imaging and biology (2023): 606-618
CD64 as novel molecular imaging marker for the characterization of synovitis in rheumatoid arthritis.
Authors: Theeuwes, Wessel F and Di Ceglie, Irene and Dorst, Daphne N and Blom, Arjen B and Bos, Desiree L and Vogl, Thomas and Tas, Sander W and Jimenez-Royo, Pilar and Bergstrom, Mats and Cleveland, Matthew and van der Kraan, Peter M and Laverman, Peter and Koenders, Marije I and van Lent, Peter L and van den Bosch, Martijn H J
Journal: Arthritis research & therapy (2023): 158
Authors: Theeuwes, Wessel F and Di Ceglie, Irene and Dorst, Daphne N and Blom, Arjen B and Bos, Desiree L and Vogl, Thomas and Tas, Sander W and Jimenez-Royo, Pilar and Bergstrom, Mats and Cleveland, Matthew and van der Kraan, Peter M and Laverman, Peter and Koenders, Marije I and van Lent, Peter L and van den Bosch, Martijn H J
Journal: Arthritis research & therapy (2023): 158
Imaging the Alternatively Spliced D Domain of Tenascin C in a Preclinical Model of Inflammatory Bowel Disease.
Authors: Zhang, Liang and Wang, Yuzhen and Homan, Kristoff T and Gaudette, Stephanie M and McCluskey, Andrew J and Chan, Ying and Murphy, Joanne and Abdalla, Mary and Nelson, Christine M and Sun, Victor Z and Erickson, Jamie E and Knight, Heather L and Clabbers, Anca and Sterman, Annette J Schwartz and Mitra, Soumya
Journal: Molecular imaging and biology (2023): 314-323
Authors: Zhang, Liang and Wang, Yuzhen and Homan, Kristoff T and Gaudette, Stephanie M and McCluskey, Andrew J and Chan, Ying and Murphy, Joanne and Abdalla, Mary and Nelson, Christine M and Sun, Victor Z and Erickson, Jamie E and Knight, Heather L and Clabbers, Anca and Sterman, Annette J Schwartz and Mitra, Soumya
Journal: Molecular imaging and biology (2023): 314-323
Identification of a Suitable Untargeted Agent for the Clinical Translation of ABY-029 Paired-Agent Imaging in Fluorescence-Guided Surgery.
Authors: Wang, Cheng and Xu, Xiaochun and Hodge, Sassan and Chen, Eunice Y and Hoopes, P Jack and Tichauer, Kenneth M and Samkoe, Kimberley S
Journal: Molecular imaging and biology (2023): 97-109
Authors: Wang, Cheng and Xu, Xiaochun and Hodge, Sassan and Chen, Eunice Y and Hoopes, P Jack and Tichauer, Kenneth M and Samkoe, Kimberley S
Journal: Molecular imaging and biology (2023): 97-109
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker.
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
In vivo quantification of programmed death-ligand-1 expression heterogeneity in tumors using fluorescence lifetime imaging.
Authors: Pal, Rahul and K, Murali and Matsui, Aya and Kang, Homan and Morita, Satoru and Taniguchi, Hajime and Kobayashi, Tatsuya and Morita, Atsuyo and Choi, Hak Soo and Duda, Dan G and Kumar, Anand T N
Journal: Research square (2023)
Authors: Pal, Rahul and K, Murali and Matsui, Aya and Kang, Homan and Morita, Satoru and Taniguchi, Hajime and Kobayashi, Tatsuya and Morita, Atsuyo and Choi, Hak Soo and Duda, Dan G and Kumar, Anand T N
Journal: Research square (2023)
Near-Infrared Fluorescence Imaging in Preclinical Models of Glioblastoma.
Authors: Llaguno-Munive, Monserrat and Villalba-Abascal, Wilberto and Avilés-Salas, Alejandro and Garcia-Lopez, Patricia
Journal: Journal of imaging (2023)
Authors: Llaguno-Munive, Monserrat and Villalba-Abascal, Wilberto and Avilés-Salas, Alejandro and Garcia-Lopez, Patricia
Journal: Journal of imaging (2023)
Preclinical evaluation of EpCAM-binding designed ankyrin repeat proteins (DARPins) as targeting moieties for bimodal near-infrared fluorescence and photoacoustic imaging of cancer.
Authors: Houvast, Ruben D and Badr, Nada and March, Taryn and de Muynck, Lysanne D A N and Sier, Vincent Q and Schomann, Timo and Bhairosingh, Shadhvi and Baart, Victor M and Peeters, Judith A H M and van Westen, Gerard J P and Plückthun, Andreas and Burggraaf, Jacobus and Kuppen, Peter J K and Vahrmeijer, Alexander L and Sier, Cornelis F M
Journal: European journal of nuclear medicine and molecular imaging (2023)
Authors: Houvast, Ruben D and Badr, Nada and March, Taryn and de Muynck, Lysanne D A N and Sier, Vincent Q and Schomann, Timo and Bhairosingh, Shadhvi and Baart, Victor M and Peeters, Judith A H M and van Westen, Gerard J P and Plückthun, Andreas and Burggraaf, Jacobus and Kuppen, Peter J K and Vahrmeijer, Alexander L and Sier, Cornelis F M
Journal: European journal of nuclear medicine and molecular imaging (2023)
Comparison of Five Near-Infrared Fluorescent Folate Conjugates in an Ovarian Cancer Model.
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155