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6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*

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Physical properties
Molecular weight680.06
Spectral properties
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)533
Emission (nm)559
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM147.046 µL735.229 µL1.47 mL7.352 mL14.705 mL
5 mM29.409 µL147.046 µL294.092 µL1.47 mL2.941 mL
10 mM14.705 µL73.523 µL147.046 µL735.229 µL1.47 mL

Molarity calculator

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Spectral properties

Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)533
Emission (nm)559



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DNaasikontaminaation m{\"a}{\"a}ritysmenetelm{\"a}n karakterisointi
Authors: Sn{\"a}ll, Juho
Journal: (2019)


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