6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] Single Isomer

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Physical properties

Molecular weight	680.06
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

2129651-79-0

Molecular weight

680.06

Correction Factor (280 nm)

0.15

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

533

Emission (nm)

559

6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	147.046 µL	735.229 µL	1.47 mL	7.352 mL	14.705 mL
5 mM	29.409 µL	147.046 µL	294.092 µL	1.47 mL	2.941 mL
10 mM	14.705 µL	73.523 µL	147.046 µL	735.229 µL	1.47 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	-
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-HEX azide	533	559	73000	0.15
6-HEX alkyne	533	559	73000	0.15
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.214
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.178
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] CAS#: 216699-36-4	578	604	82000	0.168
6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite]	533	559	73000	0.15
6-ROXtra™ SE Superior 6-ROX Replacement	578	595	-	-
6-VIC, SE [6-VIC NHS ester]	526	543	-	-
6-NED, SE [6-NED NHS ester]	545	567	74000¹	-
6-HEX acid	533	559	73000	0.15
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Images

Figure 1. Chemical structure for 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*

Citations

View all 1 citations: Citation Explorer

DNaasikontaminaation m{\"a}{\"a}ritysmenetelm{\"a}n karakterisointi
Authors: Sn{\"a}ll, Juho
Journal: (2019)

References

View all 12 references: Citation Explorer

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