6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 680.06 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 533 |
Emission (nm) | 559 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, Digital PCR, Fluoresceins, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
CAS 2129651-79-0 | Molecular weight 680.06 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 533 | Emission (nm) 559 |
6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 147.046 µL | 735.229 µL | 1.47 mL | 7.352 mL | 14.705 mL |
5 mM | 29.409 µL | 147.046 µL | 294.092 µL | 1.47 mL | 2.941 mL |
10 mM | 14.705 µL | 73.523 µL | 147.046 µL | 735.229 µL | 1.47 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 533 |
Emission (nm) | 559 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* | 493 | 517 | 83000 | 0.178 |
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3* | 520 | 545 | 750001 | - |
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] | 521 | 542 | 73000 | 0.13 |
6-HEX azide | 533 | 559 | 73000 | 0.15 |
6-HEX alkyne | 533 | 559 | 73000 | 0.15 |
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer* | 522 | 546 | 94000 | 0.214 |
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.178 |
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* | 578 | 604 | 82000 | 0.168 |
6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite] | 533 | 559 | 73000 | 0.15 |
Show More (4) |
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Citations
View all 1 citations: Citation Explorer
DNaasikontaminaation m{\"a}{\"a}ritysmenetelm{\"a}n karakterisointi
Authors: Sn{\"a}ll, Juho
Journal: (2019)
Authors: Sn{\"a}ll, Juho
Journal: (2019)
References
View all 12 references: Citation Explorer
Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization
Authors: Reid SL, Parry D, Liu HH, Connolly BA.
Journal: Biochemistry (2001): 2484
Authors: Reid SL, Parry D, Liu HH, Connolly BA.
Journal: Biochemistry (2001): 2484
Strand-separating activity of hepatitis C virus helicase in the absence of ATP
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941
Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)