6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] Single Isomer

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Physical properties

Molecular weight	680.06
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

2129651-79-0

Molecular weight

680.06

Correction Factor (280 nm)

0.15

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

533

Emission (nm)

559

6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	147.046 µL	735.229 µL	1.47 mL	7.352 mL	14.705 mL
5 mM	29.409 µL	147.046 µL	294.092 µL	1.47 mL	2.941 mL
10 mM	14.705 µL	73.523 µL	147.046 µL	735.229 µL	1.47 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	-
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-HEX azide	533	559	73000	0.15
6-HEX alkyne	533	559	73000	0.15
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.214
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.178
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] CAS#: 216699-36-4	578	604	82000	0.168
6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite]	533	559	73000	0.15
6-ROXtra™ SE Superior 6-ROX Replacement	578	595	-	-
6-VIC, SE [6-VIC NHS ester]	526	543	-	-
6-NED, SE [6-NED NHS ester]	545	567	74000¹	-
6-HEX acid	533	559	73000	0.15
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Images

Figure 1. Chemical structure for 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*

Citations

View all 1 citations: Citation Explorer

DNaasikontaminaation m{\"a}{\"a}ritysmenetelm{\"a}n karakterisointi
Authors: Sn{\"a}ll, Juho
Journal: (2019)

References

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