6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 680.06 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 533 |
Emission (nm) | 559 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Cell Structures and Organelles, Nucleus, Chemical Reagents, Classic Dyes, Dyes by Functional Group, Digital PCR, DNA and RNA Quantitation, Fluoresceins, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
CAS 2129651-79-0 | Molecular weight 680.06 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 533 | Emission (nm) 559 |
6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 147.046 µL | 735.229 µL | 1.47 mL | 7.352 mL | 14.705 mL |
5 mM | 29.409 µL | 147.046 µL | 294.092 µL | 1.47 mL | 2.941 mL |
10 mM | 14.705 µL | 73.523 µL | 147.046 µL | 735.229 µL | 1.47 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 533 |
Emission (nm) | 559 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* | 493 | 517 | 83000 | 0.178 |
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3* | 520 | 545 | 750001 | - |
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] | 521 | 542 | 73000 | 0.13 |
6-HEX azide | 533 | 559 | 73000 | 0.15 |
6-HEX alkyne | 533 | 559 | 73000 | 0.15 |
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer* | 522 | 546 | 94000 | 0.214 |
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.178 |
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* | 578 | 604 | 82000 | 0.168 |
6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite] | 533 | 559 | 73000 | 0.15 |
Show More (4) |
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Citations
View all 1 citations: Citation Explorer
DNaasikontaminaation m{\"a}{\"a}ritysmenetelm{\"a}n karakterisointi
Authors: Sn{\"a}ll, Juho
Journal: (2019)
Authors: Sn{\"a}ll, Juho
Journal: (2019)
References
View all 12 references: Citation Explorer
Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705
Authors: Iwamoto T, Sonobe T, Hayashi K.
Journal: J Clin Microbiol (2002): 4705
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization
Authors: Reid SL, Parry D, Liu HH, Connolly BA.
Journal: Biochemistry (2001): 2484
Authors: Reid SL, Parry D, Liu HH, Connolly BA.
Journal: Biochemistry (2001): 2484
Strand-separating activity of hepatitis C virus helicase in the absence of ATP
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Authors: Porter DJ, Preugschat F.
Journal: Biochemistry (2000): 5166
Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941
Authors: Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: J Clin Microbiol (1999): 1941
Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692
Authors: Gumbs OH, Shaner SL.
Journal: Biochemistry (1998): 11692
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)