Cal-590™, potassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3® filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.

Fluorescence emission spectra of Cal-590™ in solution containing 0 to 39 µM free Ca<sup>2+</sup>.

Protocol

COA

Catalog	Size	Price	Quantity
20518	5x50 ug	Price
20519	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-590™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.045 µL	445.224 µL	890.448 µL	4.452 mL	8.904 mL
5 mM	17.809 µL	89.045 µL	178.09 µL	890.448 µL	1.781 mL
10 mM	8.904 µL	44.522 µL	89.045 µL	445.224 µL	890.448 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1123.03 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain

Authors:

Birkner, Antje

A versatile and Open-Source rapid LED switching system for One-Photon imaging and Photo-Activation

Authors:

Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP

Journal:

Frontiers in cellular neuroscience (2019): 530

Gap junction coupling shapes the encoding of light in the developing retina

Authors:

Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B

Journal:

Current Biology (2019): 4024--4035

A versatile and open-source rapid LED switching system for one-photon imaging and photo-activation

Authors:

Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP

Journal:

Frontiers in cellular neuroscience (2018)

α V β 3 Integrin regulates astrocyte reactivity

Authors:

Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined

Journal:

Journal of Neuroinflammation (2017): 194

Physical properties

Dissociation constant (K_d, nM)	561
Molecular weight	1123.03
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	78000
Excitation (nm)	574
Emission (nm)	588
Quantum yield	0.62 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet (Catalog 20518)
Safety Data Sheet (Catalog 20519)
Certificate of Analysis

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Page updated on October 27, 2025