logo
AAT Bioquest

Cal-590™, potassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3® filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-590™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM89.045 µL445.224 µL890.448 µL4.452 mL8.904 mL
5 mM17.809 µL89.045 µL178.09 µL890.448 µL1.781 mL
10 mM8.904 µL44.522 µL89.045 µL445.224 µL890.448 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Citations

View all 8 citations: Citation Explorer
Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain
Authors: Birkner, Antje
Journal: (2019)
A versatile and Open-Source rapid LED switching system for One-Photon imaging and Photo-Activation
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2019): 530
Gap junction coupling shapes the encoding of light in the developing retina
Authors: Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B
Journal: Current Biology (2019): 4024--4035
A versatile and open-source rapid LED switching system for one-photon imaging and photo-activation
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2018)
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
Page updated on December 6, 2024

Ordering information

Price
Unit size
5x50 ug
1 mg
Catalog Number
2051820519
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Dissociation constant (Kd, nM)561

Molecular weight

1123.03

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

78000

Excitation (nm)

574

Emission (nm)

588

Quantum yield

0.621

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence emission spectra of Cal-590™ in solution containing 0 to 39 µM free Ca<sup>2+</sup>.
Fluorescence emission spectra of Cal-590™ in solution containing 0 to 39 µM free Ca<sup>2+</sup>.
Fluorescence emission spectra of Cal-590™ in solution containing 0 to 39 µM free Ca<sup>2+</sup>.
Ca2+ Dependent Dose Response of Cal-590.