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Cal-590™, potassium salt
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3® filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.
Fluorescence emission spectra of Cal-590™ in solution containing 0 to 39 µM free Ca<sup>2+</sup>.
Fluorescence emission spectra of Cal-590™ in solution containing 0 to 39 µM free Ca<sup>2+</sup>.
CatalogSize
Price
Quantity
205185x50 ug
Price
205191 mg
Price
 
Physical properties

Dissociation constant (Kd, nM)561
Molecular weight1123.03
SolventWater
Spectral properties

Extinction coefficient (cm -1 M -1)
78000
Excitation (nm)574
Emission (nm)588
Quantum yield
0.62
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 6, 2025