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Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments. It is designed to calibrate the real-time PCR instrument, which measures the fluorescence signal emitted by the Cy5 dye during the PCR reaction. The Cy5 dye qPCR calibration plate contains a set of pre-determined concentrations of a DNA template that serves as a reference for the quantification of unknown samples. The plate is loaded into the PCR instrument along with the unknown samples, and the instrument measures the fluorescence signal from each well during the PCR amplification process. By comparing the fluorescence signal of the unknown samples to the known concentrations in the calibration plate, researchers can determine the amount of DNA present in the unknown samples. This information can be used to quantify gene expression levels, detect the presence of pathogens, or perform other types of molecular analysis.

Spectrum

References

View all 50 references: Citation Explorer
Evaluating Novel Quantification Methods for Infectious Baculoviruses.
Authors: Lothert, Keven and Bagrin, Elena and Wolff, Michael W
Journal: Viruses (2023)
Phenol red as electrochemical indicator for highly sensitive quantification of SARS-CoV-2 by loop-mediated isothermal amplification detection.
Authors: Rioboó-Legaspi, P and González-López, A and Beltrán-Sánchez, J F and Cima-Cabal, M D and García-Suárez, M M and Sánchez, A J García and Fernández-Otero, T and Haro, J García and Costa-Rama, E and Fernández-Abedul, M T
Journal: Talanta (2023): 124963
Identification of diagnostic hub genes related to neutrophils and infiltrating immune cell alterations in idiopathic pulmonary fibrosis.
Authors: Lin, Yingying and Lai, Xiaofan and Huang, Shaojie and Pu, Lvya and Zeng, Qihao and Wang, Zhongxing and Huang, Wenqi
Journal: Frontiers in immunology (2023): 1078055
A machine learning approach to predicting 30-day mortality following paediatric cardiac surgery: findings from the Australia New Zealand Congenital Outcomes Registry for Surgery (ANZCORS).
Authors: Betts, Kim S and Marathe, Supreet P and Chai, Kevin and Konstantinov, Igor and Iyengar, Ajay and Suna, Jessica and , and Venugopal, Prem and Alphonso, Nelson
Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery (2023)
Novel m7G-related lncRNA signature for predicting overall survival in patients with gastric cancer.
Authors: Zhao, Bin and Fang, Fang and Liao, Yiqun and Chen, Yuji and Wang, Fei and Ma, Yichao and Wei, Chen and Zhao, Jiahao and Ji, Hao and Wang, Daorong and Tang, Dong
Journal: BMC bioinformatics (2023): 100
Page updated on October 8, 2024

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Catalog Number67016
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Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure