VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 526 |
Emission (nm) | 543 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Real-Time PCR (qPCR)
Molecular weight N/A | Excitation (nm) 526 | Emission (nm) 543 |
VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
VIC Dye qPCR Calibration Solution *10,000X* | 526 | 543 | - |
Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
References
View all 23 references: Citation Explorer
Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.
Authors: Perrine-Walker, Francine and Payne, Jennifer
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2021): 1077-1086
Authors: Perrine-Walker, Francine and Payne, Jennifer
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2021): 1077-1086
The P2X7 receptor antagonist JNJ-47965567 administered thrice weekly from disease onset does not alter progression of amyotrophic lateral sclerosis in SOD1G93A mice.
Authors: Ly, Diane and Dongol, Anjila and Cuthbertson, Peter and Guy, Thomas V and Geraghty, Nicholas J and Sophocleous, Reece A and Sin, Lucia and Turner, Bradley J and Watson, Debbie and Yerbury, Justin J and Sluyter, Ronald
Journal: Purinergic signalling (2020): 109-122
Authors: Ly, Diane and Dongol, Anjila and Cuthbertson, Peter and Guy, Thomas V and Geraghty, Nicholas J and Sophocleous, Reece A and Sin, Lucia and Turner, Bradley J and Watson, Debbie and Yerbury, Justin J and Sluyter, Ronald
Journal: Purinergic signalling (2020): 109-122
Development of the MitoQ assay as a real-time quantification of mitochondrial DNA in degraded samples.
Authors: Wai, Ka Tak and Gunn, Peter and Barash, Mark
Journal: International journal of legal medicine (2019): 411-417
Authors: Wai, Ka Tak and Gunn, Peter and Barash, Mark
Journal: International journal of legal medicine (2019): 411-417
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Lineage-specific detection of influenza B virus using real-time polymerase chain reaction with melting curve analysis.
Authors: Tewawong, Nipaporn and Chansaenroj, Jira and Klinfueng, Sirapa and Vichiwattana, Preeyaporn and Korkong, Sumeth and Thongmee, Thanunrat and Theamboonlers, Apiradee and Payungporn, Sunchai and Vongpunsawad, Sompong and Poovorawan, Yong
Journal: Archives of virology (2016): 1425-35
Authors: Tewawong, Nipaporn and Chansaenroj, Jira and Klinfueng, Sirapa and Vichiwattana, Preeyaporn and Korkong, Sumeth and Thongmee, Thanunrat and Theamboonlers, Apiradee and Payungporn, Sunchai and Vongpunsawad, Sompong and Poovorawan, Yong
Journal: Archives of virology (2016): 1425-35
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression.
Authors: Wang, Yiming and Zhang, Hongming and Chai, Fangxian and Liu, Xingde and Berk, Michael
Journal: BMC psychiatry (2014): 349
Authors: Wang, Yiming and Zhang, Hongming and Chai, Fangxian and Liu, Xingde and Berk, Michael
Journal: BMC psychiatry (2014): 349
Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal β-like globin genes.
Authors: Roosjen, Mark and McColl, Bradley and Kao, Betty and Gearing, Linden J and Blewitt, Marnie E and Vadolas, Jim
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014): 1610-20
Authors: Roosjen, Mark and McColl, Bradley and Kao, Betty and Gearing, Linden J and Blewitt, Marnie E and Vadolas, Jim
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014): 1610-20
Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.
Authors: Naito, Yoshiki and Tomita, Motonori
Journal: Biochemical genetics (2013): 530-42
Authors: Naito, Yoshiki and Tomita, Motonori
Journal: Biochemical genetics (2013): 530-42
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?