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VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

ABI 7500 FAST system VIC dye spectrum
ABI 7500 FAST system VIC dye spectrum
ABI 7500 FAST system VIC dye spectrum
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Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)526
Emission (nm)543
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Excitation (nm)
526
Emission (nm)
543
VIC Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.

Spectrum


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spectrum

Spectral properties

Excitation (nm)526
Emission (nm)543

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References


View all 23 references: Citation Explorer
Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.
Authors: Perrine-Walker, Francine and Payne, Jennifer
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2021): 1077-1086
The P2X7 receptor antagonist JNJ-47965567 administered thrice weekly from disease onset does not alter progression of amyotrophic lateral sclerosis in SOD1G93A mice.
Authors: Ly, Diane and Dongol, Anjila and Cuthbertson, Peter and Guy, Thomas V and Geraghty, Nicholas J and Sophocleous, Reece A and Sin, Lucia and Turner, Bradley J and Watson, Debbie and Yerbury, Justin J and Sluyter, Ronald
Journal: Purinergic signalling (2020): 109-122
Development of the MitoQ assay as a real-time quantification of mitochondrial DNA in degraded samples.
Authors: Wai, Ka Tak and Gunn, Peter and Barash, Mark
Journal: International journal of legal medicine (2019): 411-417
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Lineage-specific detection of influenza B virus using real-time polymerase chain reaction with melting curve analysis.
Authors: Tewawong, Nipaporn and Chansaenroj, Jira and Klinfueng, Sirapa and Vichiwattana, Preeyaporn and Korkong, Sumeth and Thongmee, Thanunrat and Theamboonlers, Apiradee and Payungporn, Sunchai and Vongpunsawad, Sompong and Poovorawan, Yong
Journal: Archives of virology (2016): 1425-35
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression.
Authors: Wang, Yiming and Zhang, Hongming and Chai, Fangxian and Liu, Xingde and Berk, Michael
Journal: BMC psychiatry (2014): 349
Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal β-like globin genes.
Authors: Roosjen, Mark and McColl, Bradley and Kao, Betty and Gearing, Linden J and Blewitt, Marnie E and Vadolas, Jim
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014): 1610-20
Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.
Authors: Naito, Yoshiki and Tomita, Motonori
Journal: Biochemical genetics (2013): 530-42