NED Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Absorbance (nm)	544
Extinction coefficient (cm ^-1 M ^-1)	74000¹
Excitation (nm)	545
Emission (nm)	567

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	544
Extinction coefficient (cm ^-1 M ^-1)	74000¹
Excitation (nm)	545
Emission (nm)	567

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
TAMRA Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	552	578	90000
VIC Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	526	543	-
ROX Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	578	604	82000
FAM Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	493	517	83000
SYBR Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	498	522	-
JOE Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	520	545	75000¹
NED Dye qPCR Calibration Solution 10,000X	545	567	74000¹
Cy5 Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	651	670	250000¹

Images

Figure 1. ABI 7500 FAST system NED dye spectrum

References

View all 12 references: Citation Explorer

DNA degradation in human teeth exposed to thermal stress.
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22

Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57

An improved rapid quantitative detection and identification method for a wide range of fungi.
Authors: Soeta, Nobutoshi and Terashima, Masanori and Gotoh, Mitsukazu and Mori, Shuichi and Nishiyama, Kyoko and Ishioka, Ken and Kaneko, Hisatoshi and Suzutani, Tatsuo
Journal: Journal of medical microbiology (2009): 1037-1044

Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36

TaqMan reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Medeiros, L Jeffrey
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 135-45

TaqMan RT-PCR assay coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Sanchez-Vega, Beatriz and Medeiros, L Jeffrey
Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2004): 96-103

Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses.
Authors: Dimsoski, Pero
Journal: Croatian medical journal (2003): 332-5

Semiquantitative and qualitative assessment of B-lymphocyte V H repertoire by a fluorescent multiplex PCR.
Authors: Feuchtenberger, Martin and Tony, Hans-Peter and Rouzière, Anne-Sophie and Jacobi, Anette and Dörner, Thomas and Kneitz, Christian and Starostik, Petr
Journal: Journal of immunological methods (2003): 121-7