SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 498 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Real-Time PCR (qPCR)
Molecular weight N/A | Excitation (nm) 498 | Emission (nm) 522 |
SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
References
View all 50 references: Citation Explorer
Combined evaluation of proliferation and apoptosis to calculate IC50 of VPA-induced PANC-1 cells and assessing its effect on the Wnt signaling pathway.
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109
Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays.
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)
Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias.
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors.
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Preoperative heat shock protein 47 levels identify colorectal cancer patients with lymph node metastasis and poor prognosis.
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources.
Authors: Wang, Fu-Der and Wu, Ping-Feng and Chen, Su-Jung
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2019): 426-432
Authors: Wang, Fu-Der and Wu, Ping-Feng and Chen, Su-Jung
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2019): 426-432
Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance.
Authors: Saeed, Noor AlHuda Ali A H and Hamzah, Israa Hussein and Al-Gharrawi, Samar Abdul Raheem
Journal: BMC research notes (2019): 455
Authors: Saeed, Noor AlHuda Ali A H and Hamzah, Israa Hussein and Al-Gharrawi, Samar Abdul Raheem
Journal: BMC research notes (2019): 455
Aberrant Expression of the miR-181b/miR-222 after Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia.
Authors: Iravani Saadi, Mahdiyar and Arandi, Nargess and Yaghobi, Ramin and Azarpira, Negar and Geramizadeh, Bita and Ramzi, Mani
Journal: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion (2019): 446-450
Authors: Iravani Saadi, Mahdiyar and Arandi, Nargess and Yaghobi, Ramin and Azarpira, Negar and Geramizadeh, Bita and Ramzi, Mani
Journal: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion (2019): 446-450
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?