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JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

ABI 7500 FAST system JOE dye spectrum
ABI 7500 FAST system JOE dye spectrum
ABI 7500 FAST system JOE dye spectrum
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Physical properties
Molecular weightN/A
Spectral properties
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)520
Emission (nm)545
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
JOE Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.


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Spectral properties

Extinction coefficient (cm -1 M -1)750001
Excitation (nm)520
Emission (nm)545



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The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81
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Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
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Mineralization Effect of Hyaluronan on Dental Pulp Cells via CD44.
Authors: Chen, Kuan-Liang and Yeh, Ying-Yi and Lung, Jrhau and Yang, Yu-Chi and Yuan, Kuo
Journal: Journal of endodontics (2016): 711-6
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Combined effects of simvastatin and enamel matrix derivative on odontoblastic differentiation of human dental pulp cells.
Authors: Karanxha, Lorena and Park, Su-Jung and Son, Won-Jun and Nör, Jacques E and Min, Kyung-San
Journal: Journal of endodontics (2013): 76-82
In vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time PCR.
Authors: Kim, Sin-Young and Shin, Yooseok and Lee, Chan-Young and Jung, Il-Young
Journal: Journal of endodontics (2013): 1359-63
Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqMan™ probe.
Authors: Ryazantsev, Dmitry Y and Tsybulsky, Dmitry A and Prokhorenko, Igor A and Kvach, Maksim V and Martynenko, Yury V and Philipchenko, Pavel M and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Analytical and bioanalytical chemistry (2012): 59-68
ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
Authors: Zhao, Zheng and Liu, Hongchen and Wang, Dongsheng
Journal: Journal of endodontics (2011): 332-9