TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Real-Time PCR (qPCR)
Molecular weight N/A | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 552 | Emission (nm) 578 |
TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
TAMRA Dye qPCR Calibration Solution *10,000X* | 552 | 578 | 90000 |
References
View all 50 references: Citation Explorer
Development and validation of a new 18 X-STR typing assay for forensic applications.
Authors: Zhang, Yinming and Yu, Zhengliang and Mo, Xiaoting and Zhao, Xingchun and Li, Wanshui and Liu, Hong and Liu, Chao and Wu, Riga and Sun, Hongyu
Journal: Electrophoresis (2021): 766-773
Authors: Zhang, Yinming and Yu, Zhengliang and Mo, Xiaoting and Zhao, Xingchun and Li, Wanshui and Liu, Hong and Liu, Chao and Wu, Riga and Sun, Hongyu
Journal: Electrophoresis (2021): 766-773
[Mesenchymal stem cells derived apoptotic extracellular vesicles attenuate pro-inflammatory macrophages induced by Porphyromonas gingivalis lipopolysaccharide].
Authors: Ye, Q Y and Li, Z H and Wang, Y Z and Liu, S Y and Zhou, J and Liu, S Y and Wang, Q T
Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (2021): 791-798
Authors: Ye, Q Y and Li, Z H and Wang, Y Z and Liu, S Y and Zhou, J and Liu, S Y and Wang, Q T
Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (2021): 791-798
MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.
Authors: Wu, Xiaosong and Pan, Jingyu and Zhu, Xinchao and Hong, Chenggang and Hu, Anzhong and Zhu, Cancan and Liu, Yong and Yang, Ke and Zhu, Ling
Journal: The Analyst (2021): 3823-3833
Authors: Wu, Xiaosong and Pan, Jingyu and Zhu, Xinchao and Hong, Chenggang and Hu, Anzhong and Zhu, Cancan and Liu, Yong and Yang, Ke and Zhu, Ling
Journal: The Analyst (2021): 3823-3833
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Double-Stranded DNA Fragments Bearing Unrepairable Lesions and Their Internalization into Mouse Krebs-2 Carcinoma Cells.
Authors: Dolgova, Evgeniya V and Evdokimov, Alexey N and Proskurina, Anastasia S and Efremov, Yaroslav R and Bayborodin, Sergey I and Potter, Ekaterina A and Popov, Alexey A and Petruseva, Irina O and Lavrik, Olga I and Bogachev, Sergey S
Journal: Nucleic acid therapeutics (2019): 278-290
Authors: Dolgova, Evgeniya V and Evdokimov, Alexey N and Proskurina, Anastasia S and Efremov, Yaroslav R and Bayborodin, Sergey I and Potter, Ekaterina A and Popov, Alexey A and Petruseva, Irina O and Lavrik, Olga I and Bogachev, Sergey S
Journal: Nucleic acid therapeutics (2019): 278-290
Design and anti-tumor activity of self-loaded nanocarriers of siRNA.
Authors: Han, Wenzhao and Yuan, Ye and Li, Hui and Fu, Zhendong and Wang, Mingyang and Guan, Shuwen and Wang, Liping
Journal: Colloids and surfaces. B, Biointerfaces (2019): 110385
Authors: Han, Wenzhao and Yuan, Ye and Li, Hui and Fu, Zhendong and Wang, Mingyang and Guan, Shuwen and Wang, Liping
Journal: Colloids and surfaces. B, Biointerfaces (2019): 110385
The mechanism and regularity of quenching the effect of bases on fluorophores: the base-quenched probe method.
Authors: Mao, Huihui and Luo, Guanghua and Zhan, Yuxia and Zhang, Jun and Yao, Shuang and Yu, Yang
Journal: The Analyst (2018): 3292-3301
Authors: Mao, Huihui and Luo, Guanghua and Zhan, Yuxia and Zhang, Jun and Yao, Shuang and Yu, Yang
Journal: The Analyst (2018): 3292-3301
Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells.
Authors: Potter, Ekaterina A and Dolgova, Evgenia V and Proskurina, Anastasia S and Efremov, Yaroslav R and Minkevich, Alexandra M and Rozanov, Aleksey S and Peltek, Sergey E and Nikolin, Valeriy P and Popova, Nelly A and Seledtsov, Igor A and Molodtsov, Vladimir V and Zavyalov, Evgeniy L and Taranov, Oleg S and Baiborodin, Sergey I and Ostanin, Alexander A and Chernykh, Elena R and Kolchanov, Nikolay A and Bogachev, Sergey S
Journal: Oncotarget (2017): 9425-9441
Authors: Potter, Ekaterina A and Dolgova, Evgenia V and Proskurina, Anastasia S and Efremov, Yaroslav R and Minkevich, Alexandra M and Rozanov, Aleksey S and Peltek, Sergey E and Nikolin, Valeriy P and Popova, Nelly A and Seledtsov, Igor A and Molodtsov, Vladimir V and Zavyalov, Evgeniy L and Taranov, Oleg S and Baiborodin, Sergey I and Ostanin, Alexander A and Chernykh, Elena R and Kolchanov, Nikolay A and Bogachev, Sergey S
Journal: Oncotarget (2017): 9425-9441
Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods.
Authors: Stachelska, M A
Journal: Polish journal of veterinary sciences (2017): 477-484
Authors: Stachelska, M A
Journal: Polish journal of veterinary sciences (2017): 477-484
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?