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TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

ABI 7500 FAST system TAMRA dye spectrum
ABI 7500 FAST system TAMRA dye spectrum
ABI 7500 FAST system TAMRA dye spectrum
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.178
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)
552
Emission (nm)
578
TAMRA Dye qPCR Calibration Plate can be used to calibrate and maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578

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References


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Journal: The Analyst (2018): 3292-3301
Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells.
Authors: Potter, Ekaterina A and Dolgova, Evgenia V and Proskurina, Anastasia S and Efremov, Yaroslav R and Minkevich, Alexandra M and Rozanov, Aleksey S and Peltek, Sergey E and Nikolin, Valeriy P and Popova, Nelly A and Seledtsov, Igor A and Molodtsov, Vladimir V and Zavyalov, Evgeniy L and Taranov, Oleg S and Baiborodin, Sergey I and Ostanin, Alexander A and Chernykh, Elena R and Kolchanov, Nikolay A and Bogachev, Sergey S
Journal: Oncotarget (2017): 9425-9441
Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods.
Authors: Stachelska, M A
Journal: Polish journal of veterinary sciences (2017): 477-484
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
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