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AAT Bioquest

VIC Dye qPCR Calibration Solution *10,000X*

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as VIC, will depend on the device used. The AAT Bioquest VIC dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.

Prepare a VIC calibration plate:
  1. Prepare a VIC Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X VIC Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.

    Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  2. Add 20 µL of the VIC Dye qPCR Calibration working solution to all wells of a reaction plate.

    Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.

  3. Seal the plate with an optical adhesive cover.

  4. Centrifuge the plate for 2 minutes at < 1500 rpm.

  5. Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.

  6. For complete calibration instructions, refer to the maintenance guide for your instrument.

Spectrum

References

View all 50 references: Citation Explorer
The role of lateral size of MXene nanosheets in membrane filtration of dyeing wastewater: Membrane characteristic and performance.
Authors: Xiang, Jing and Wang, Xingmin and Ding, Mingmei and Tang, Xiaomin and Zhang, Shixin and Zhang, Xianming and Xie, Zongli
Journal: Chemosphere (2022): 133728
Interrogating the Impassable: A Case Series and Literature Review of Unilateral SPECT-CT Groin Visualization in Men With Penile Cancer.
Authors: O'Brien, Jonathan S and Teh, Jiasian and Kelly, Brian D and Chen, Kenneth and Manning, Todd and Furrer, Marc and Chee, Justin and Lawrentschuk, Nathan
Journal: Frontiers in surgery (2022): 882011
Multifunctional oil-produced reduced graphene oxide - Silver oxide composites with photocatalytic, antioxidant, and antibacterial activities.
Authors: Lekshmi, G S and Tamilselvi, R and Geethalakshmi, R and Kirupha, S D and Bazaka, O and Levchenko, I and Bazaka, K and Mandhakini, M
Journal: Journal of colloid and interface science (2022): 294-305
An Ultrasound-Responsive Theranostic Cyclodextrin-Loaded Nanoparticle for Multimodal Imaging and Therapy for Atherosclerosis.
Authors: Mehta, Sourabh and Bongcaron, Viktoria and Nguyen, Tien K and Jirwanka, Yugandhara and Maluenda, Ana and Walsh, Aidan P G and Palasubramaniam, Jathushan and Hulett, Mark D and Srivastava, Rohit and Bobik, Alex and Wang, Xiaowei and Peter, Karlheinz
Journal: Small (Weinheim an der Bergstrasse, Germany) (2022): e2200967
Optimising the photothrombotic model of stroke in the C57BI/6 and FVB/N strains of mouse.
Authors: Knezic, Adriana and Broughton, Brad R S and Widdop, Robert E and McCarthy, Claudia A
Journal: Scientific reports (2022): 7598
Page updated on October 12, 2024

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Catalog Number67030
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Physical properties

Solvent

Water

Spectral properties

Excitation (nm)

526

Emission (nm)

543

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

Platform

qPCR

Recommended platePCR Microplate
Instrument specification(s)VIC Filter
ABI 7500 FAST system VIC dye spectrum.
ABI 7500 FAST system VIC dye spectrum.
ABI 7500 FAST system VIC dye spectrum.