VIC Dye qPCR Calibration Solution 10,000X

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Solvent

Water

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Platform

qPCR

Recommended plate	PCR Microplate
Instrument specification(s)	VIC Filter

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.

Prepare a VIC calibration plate:

Prepare a VIC Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X VIC Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.

Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the VIC Dye qPCR Calibration working solution to all wells of a reaction plate.

Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For complete calibration instructions, refer to the maintenance guide for your instrument.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
VIC Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well	526	543	-
NED Dye qPCR Calibration Solution 10,000X	545	567	74000¹
FAM Dye qPCR Calibration Solution 10,000X	493	517	83000
Cy5 Dye qPCR Calibration Solution 10,000X	651	670	250000¹
ROX Dye qPCR Calibration Solution 10,000X	578	595	-
SYBR Dye qPCR Calibration Solution 10,000X	498	522	-
TAMRA Dye qPCR Calibration Solution 10,000X	552	578	90000
ABY Dye qPCR Calibration Solution 10,000X	559	570	120000¹
JUN Dye qPCR Calibration Solution 10,000X	610	628	110000¹
MP650 Dye qPCR Calibration Solution 10,000X	639	653	250000¹
Show More (1)

Images

Figure 1. ABI 7500 FAST system VIC dye spectrum.

References

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The role of lateral size of MXene nanosheets in membrane filtration of dyeing wastewater: Membrane characteristic and performance.
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Authors: Park, Inkyung and Oh, Sungtaek and Nam, Hyoyoun and Celi, Pietro and Lillehoj, Hyun S
Journal: Poultry science (2022): 101731

Bioremediation of Industrial Pollutants by Insects Expressing a Fungal Laccase.
Authors: Clark, Michael and Tepper, Kate and Petroll, Kerstin and Kumar, Sheemal and Sunna, Anwar and Maselko, Maciej
Journal: ACS synthetic biology (2022): 308-316

Identifying adsorption sites for Cd(II) and organic dyes on modified straw materials.
Authors: Liu, Wei and Huang, Jie and Weatherley, Anthony J and Zhai, Wenjun and Liu, Fuya and Ma, Zhiling and Jiao, Yunhong and Zhang, Chao and Han, Bing
Journal: Journal of environmental management (2022): 113862

Bottlebrush copolymers for gene delivery: influence of architecture, charge density, and backbone length on transfection efficiency.
Authors: Floyd, Thomas G and Song, Ji-Inn and Hapeshi, Alexia and Laroque, Sophie and Hartlieb, Matthias and Perrier, Sébastien
Journal: Journal of materials chemistry. B (2022): 3696-3704

Optimising the photothrombotic model of stroke in the C57BI/6 and FVB/N strains of mouse.
Authors: Knezic, Adriana and Broughton, Brad R S and Widdop, Robert E and McCarthy, Claudia A
Journal: Scientific reports (2022): 7598

An Ultrasound-Responsive Theranostic Cyclodextrin-Loaded Nanoparticle for Multimodal Imaging and Therapy for Atherosclerosis.
Authors: Mehta, Sourabh and Bongcaron, Viktoria and Nguyen, Tien K and Jirwanka, Yugandhara and Maluenda, Ana and Walsh, Aidan P G and Palasubramaniam, Jathushan and Hulett, Mark D and Srivastava, Rohit and Bobik, Alex and Wang, Xiaowei and Peter, Karlheinz
Journal: Small (Weinheim an der Bergstrasse, Germany) (2022): e2200967