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MP650 Dye qPCR Calibration Solution *10,000X*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 639 |
| Emission (nm) | 653 |
| Quantum yield | 0.291 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 639 | Emission (nm) 653 | Quantum yield 0.291 |
MP650 is designed to have identical spectra to those of Mustang Purple. Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as MP650, will depend on the device used. The AAT Bioquest MP650 dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.
Platform
qPCR
| Recommended plate | PCR Microplate |
| Instrument specification(s) | MP650 or Mustang Purple Filter |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.
Prepare a MP650 calibration plate:
Prepare an MP650 Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X MP650 Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.
Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the MP650 Dye qPCR Calibration working solution to all wells of a reaction plate.
Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For complete calibration instructions, refer to the maintenance guide for your instrument.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 639 |
| Emission (nm) | 653 |
| Quantum yield | 0.291 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| VIC Dye qPCR Calibration Solution *10,000X* | 526 | 543 | - |
| NED Dye qPCR Calibration Solution *10,000X* | 545 | 567 | 740001 |
| FAM Dye qPCR Calibration Solution *10,000X* | 493 | 517 | 83000 |
| Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
| ROX Dye qPCR Calibration Solution *10,000X* | 578 | 595 | - |
| SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
| TAMRA Dye qPCR Calibration Solution *10,000X* | 552 | 578 | 90000 |
| ABY Dye qPCR Calibration Solution *10,000X* | 559 | 570 | 1200001 |
| JUN Dye qPCR Calibration Solution *10,000X* | 610 | 628 | 1100001 |
Show More (1) | |||
Images
Figure 1. ABI 7500 FAST system MP650 dye spectrum.
References
View all 24 references: Citation Explorer
Detection of Sulfoquinovosidase Activity in Cell Lysates Using Activity-Based Probes.
Authors: Li, Zirui and Pickles, Isabelle and Sharma, Mahima and Melling, Benjamin and Codee, Jeroen and Pallasdies, Luise and Williams, Spencer and Overkleeft, Herman and Davies, Gideon John
Journal: Angewandte Chemie (International ed. in English) (2024): e202401358
Authors: Li, Zirui and Pickles, Isabelle and Sharma, Mahima and Melling, Benjamin and Codee, Jeroen and Pallasdies, Luise and Williams, Spencer and Overkleeft, Herman and Davies, Gideon John
Journal: Angewandte Chemie (International ed. in English) (2024): e202401358
Toxicity Studies of Cardiac-Targeting Peptide Reveal a Robust Safety Profile.
Authors: Sahagun, Daniella A and Lopuszynski, Jack B and Feldman, Kyle S and Pogodzinski, Nicholas and Zahid, Maliha
Journal: Pharmaceutics (2024)
Authors: Sahagun, Daniella A and Lopuszynski, Jack B and Feldman, Kyle S and Pogodzinski, Nicholas and Zahid, Maliha
Journal: Pharmaceutics (2024)
Analytical sensitivity and clinical performance of "COVID-19 RT-PCR Real TM FAST (CY5) (ATGen, Uruguay) and "ECUGEN SARS-CoV-2 RT-qPCR" (UDLA-STARNEWCORP, Ecuador)": High quality-low cost local SARS-CoV-2 tests for South America.
Authors: Freire-Paspuel, Byron and Morales-Jadan, Diana and Zambrano-Mila, Marlon and Perez, Franklin and Garcia-Bereguiain, Miguel Angel
Journal: PLoS neglected tropical diseases (2022): e0010082
Authors: Freire-Paspuel, Byron and Morales-Jadan, Diana and Zambrano-Mila, Marlon and Perez, Franklin and Garcia-Bereguiain, Miguel Angel
Journal: PLoS neglected tropical diseases (2022): e0010082
Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA.
Authors: Kekić, Tadija and Lietard, Jory
Journal: Scientific reports (2022): 14803
Authors: Kekić, Tadija and Lietard, Jory
Journal: Scientific reports (2022): 14803
Multiplex qPCR for differentiation of Mycobacterium avium subspecies paratuberculosis in active and passive infection of goats.
Authors: Pachoori, Anjali and Gururaj, K and Sachan, Supriya and Sharma, Deepansh
Journal: Applied microbiology and biotechnology (2022): 4705-4717
Authors: Pachoori, Anjali and Gururaj, K and Sachan, Supriya and Sharma, Deepansh
Journal: Applied microbiology and biotechnology (2022): 4705-4717
Comparative performances of seven quantitative Reverse-Transcription Polymerase Chain Reaction assays (RT-qPCR) for detecting SARS-CoV-2 infection in samples from individuals suspected of COVID-19 in São Paulo, Brazil.
Authors: Fukasawa, Lucila Okuyama and Sacchi, Cláudio Tavares and Gonçalves, Maria Gisele and Lemos, Ana Paula Silva and Almeida, Samanta Cristine Grassi and Caterino-de-Araujo, Adele
Journal: Journal of clinical virology plus (2021): 100012
Authors: Fukasawa, Lucila Okuyama and Sacchi, Cláudio Tavares and Gonçalves, Maria Gisele and Lemos, Ana Paula Silva and Almeida, Samanta Cristine Grassi and Caterino-de-Araujo, Adele
Journal: Journal of clinical virology plus (2021): 100012
Biomimetic, ROS-detonable nanoclusters - A multimodal nanoplatform for anti-restenotic therapy.
Authors: Zhao, Yi and Shirasu, Takuro and Yodsanit, Nisakorn and Kent, Eric and Ye, Mingzhou and Wang, Yuyuan and Xie, Ruosen and Gregg, Alexander Christopher and Huang, Yitao and Kent, K Craig and Guo, Lian-Wang and Gong, Shaoqin and Wang, Bowen
Journal: Journal of controlled release : official journal of the Controlled Release Society (2021): 295-306
Authors: Zhao, Yi and Shirasu, Takuro and Yodsanit, Nisakorn and Kent, Eric and Ye, Mingzhou and Wang, Yuyuan and Xie, Ruosen and Gregg, Alexander Christopher and Huang, Yitao and Kent, K Craig and Guo, Lian-Wang and Gong, Shaoqin and Wang, Bowen
Journal: Journal of controlled release : official journal of the Controlled Release Society (2021): 295-306
Analytical sensitivity and clinical performance of a triplex RT-qPCR assay using CDC N1, N2, and RP targets for SARS-CoV-2 diagnosis.
Authors: Freire-Paspuel, Byron and Garcia-Bereguiain, Miguel Angel
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2021): 14-16
Authors: Freire-Paspuel, Byron and Garcia-Bereguiain, Miguel Angel
Journal: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (2021): 14-16
The effects of dithiothreitol (DTT) on fluorescent qPCR dyes.
Authors: Hudson, Brittany C and Cox, Jordan O and Seashols-Williams, Sarah J and Dawson Cruz, Tracey
Journal: Journal of forensic sciences (2021): 700-708
Authors: Hudson, Brittany C and Cox, Jordan O and Seashols-Williams, Sarah J and Dawson Cruz, Tracey
Journal: Journal of forensic sciences (2021): 700-708
Integration of Polylactide into Polyethylenimine Facilitates the Safe and Effective Intracellular siRNA Delivery.
Authors: Ding, Guo-Bin and Meng, Xue and Yang, Peng and Li, Binchun and Stauber, Roland H and Li, Zhuoyu
Journal: Polymers (2020)
Authors: Ding, Guo-Bin and Meng, Xue and Yang, Peng and Li, Binchun and Stauber, Roland H and Li, Zhuoyu
Journal: Polymers (2020)