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D-Luciferin phosphate *CAS 145613-12-3*

Chemical structure for D-Luciferin phosphate *CAS 145613-12-3*
Chemical structure for D-Luciferin phosphate *CAS 145613-12-3*
Ordering information
Price ()
Catalog Number12512
Unit Size
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Additional ordering information
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Physical properties
Molecular weight426.25
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41105331

OverviewpdfSDSpdfProtocol


CAS
145613-12-3
Molecular weight
426.25
D-Luciferin phosphate is a highly sensitive bioluminescent substrate for detecting alkaline phosphatases and protein phosphatases by luminometric detection. It is used in combination with luciferase/ATP system. D-Luciferin phosphate can be converted to D-Luciferin by phosphatases (such as alkaline phosphatases, acid phosphatases and protein phosphatases) that gives luminescence in the presence of luciferase and its cofactors. Our Amplite luciferase assay kit can be used for detecting D-luciferin release from D-Luciferin phosphate by phosphatases.

Example protocol


AT A GLANCE

Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt.

PREPARATION OF WORKING SOLUTION

Working solution should be prepared in water or DMSO.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin phosphate *CAS 145613-12-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM234.604 µL1.173 mL2.346 mL11.73 mL23.46 mL
5 mM46.921 µL234.604 µL469.208 µL2.346 mL4.692 mL
10 mM23.46 µL117.302 µL234.604 µL1.173 mL2.346 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Citations


View all 2 citations: Citation Explorer
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

References


View all 22 references: Citation Explorer
Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI
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Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface
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Journal: Nature (2004): 465
Novel bioluminescent assay of alkaline phosphatase using adenosine-3'-phosphate-5'-phosphosulfate as substrate and the luciferin-luciferase reaction and its application
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Journal: Anal Biochem (2003): 206
Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26
Authors: Braet K, V and amme W, Martin PE, Evans WH, Leybaert L.
Journal: Cell Calcium (2003): 37
Mitochondrial membrane potential and intracellular ATP content after transient experimental ischemia in the cultured hippocampal neuron
Authors: Iijima T, Mishima T, Tohyama M, Akagawa K, Iwao Y.
Journal: Neurochem Int (2003): 263
Highly sensitive simultaneous bioluminescent measurement of acetate kinase and pyruvate phosphate dikinase activities using a firefly luciferase-luciferin reaction and its application to a tandem bioluminescent enzyme immunoassay
Authors: Ito K, Nakagawa K, Murakami S, Arakawa H, Maeda M.
Journal: Anal Sci (2003): 105
ATP released from astrocytes during swelling activates chloride channels
Authors: Darby M, Kuzmiski JB, Panenka W, Feighan D, MacVicar BA.
Journal: J Neurophysiol (2003): 1870