D-Luciferin phosphate *CAS 145613-12-3*
D-Luciferin phosphate is a highly sensitive bioluminescent substrate for detecting alkaline phosphatases and protein phosphatases by luminometric detection. It is used in combination with luciferase/ATP system. D-Luciferin phosphate can be converted to D-Luciferin by phosphatases (such as alkaline phosphatases, acid phosphatases and protein phosphatases) that gives luminescence in the presence of luciferase and its cofactors. Our Amplite luciferase assay kit can be used for detecting D-luciferin release from D-Luciferin phosphate by phosphatases.
Example protocol
AT A GLANCE
Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.
It can be used within 12 months from the date of receipt.
PREPARATION OF WORKING SOLUTION
Working solution should be prepared in water or DMSO.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of D-Luciferin phosphate *CAS 145613-12-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 234.604 µL | 1.173 mL | 2.346 mL | 11.73 mL | 23.46 mL |
5 mM | 46.921 µL | 234.604 µL | 469.208 µL | 2.346 mL | 4.692 mL |
10 mM | 23.46 µL | 117.302 µL | 234.604 µL | 1.173 mL | 2.346 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Product family
Name | Excitation (nm) | Emission (nm) |
D-Luciferin galactoside *CAS 131474-38-9* | 385 | 529 |
D-Luciferin acetate | 385 | 529 |
Citations
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Journal: Journal of Extracellular Vesicles (2024): e12490
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Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
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Journal: Plant Physiology (2023): kiad445
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Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
References
View all 22 references: Citation Explorer
Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI
Authors: Zhao D, Richer E, Antich PP, Mason RP.
Journal: Faseb J. (2008)
Authors: Zhao D, Richer E, Antich PP, Mason RP.
Journal: Faseb J. (2008)
Mechanism of the stress-induced collapse of the Ran distribution
Authors: Yasuda Y, Miyamoto Y, Saiwaki T, Yoneda Y.
Journal: Exp Cell Res (2006): 512
Authors: Yasuda Y, Miyamoto Y, Saiwaki T, Yoneda Y.
Journal: Exp Cell Res (2006): 512
Creatine phosphate administration preserves myocardial function in a model of off-pump coronary revascularization
Authors: Woo YJ, Gr and TJ, Zentko S, Cohen JE, Hsu V, Atluri P, Berry MF, Taylor MD, Moise MA, Fisher O, Kolakowski S.
Journal: J Cardiovasc Surg (Torino) (2005): 297
Authors: Woo YJ, Gr and TJ, Zentko S, Cohen JE, Hsu V, Atluri P, Berry MF, Taylor MD, Moise MA, Fisher O, Kolakowski S.
Journal: J Cardiovasc Surg (Torino) (2005): 297
Human keratinocytes release ATP and utilize three mechanisms for nucleotide interconversion at the cell surface
Authors: Burrell HE, Wlodarski B, Foster BJ, Buckley KA, Sharpe GR, Quayle JM, Simpson AW, Gallagher JA.
Journal: J Biol Chem (2005): 29667
Authors: Burrell HE, Wlodarski B, Foster BJ, Buckley KA, Sharpe GR, Quayle JM, Simpson AW, Gallagher JA.
Journal: J Biol Chem (2005): 29667
Mechanically driven ATP synthesis by F1-ATPase
Authors: Itoh H, Takahashi A, Adachi K, Noji H, Yasuda R, Yoshida M, Kinosita K.
Journal: Nature (2004): 465
Authors: Itoh H, Takahashi A, Adachi K, Noji H, Yasuda R, Yoshida M, Kinosita K.
Journal: Nature (2004): 465
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