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ReadiLink™ xtra Rapid iFluor® 350 Antibody Labeling Kit
BSA-Compatible
ReadiLink™ xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed. Preactivated iFluor® 350 used in this ReadiLink™ kit is quite stable and shows good reactivity and selectivity with antibodies. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of preactivated iFluor® 350 dye provided in the kit is optimized for labeling ~50 µg antibody. ReadiLink™ xtra iFluor® 350 rapid antibody labeling kit provides a convenient and robust method to label monoclonal and polyclonal antibodies with the bright blue fluorescent iFluor® 350 fluorophore. AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm).

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ xtra Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with rabbit anti-tubulin monoclonal antibody and stained with a goat anti-rabbit IgG labeled using the ReadiLink™ xtra Rapid iFluor® 350 Antibody Labeling Kit (Cat No. 1950).
Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with rabbit anti-tubulin monoclonal antibody and stained with a goat anti-rabbit IgG labeled using the ReadiLink™ xtra Rapid iFluor® 350 Antibody Labeling Kit (Cat No. 1950).
CatalogSize
Price
Quantity
19502 Labelings
Price
 
Spectral properties

Correction factor (260 nm)0.83
Correction factor (280 nm)0.23
Extinction coefficient (cm -1 M -1)
20000
1
Excitation (nm)345
Emission (nm)450
Quantum yield
0.95
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 8, 2025