ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Correction Factor (260 nm) | 0.044 |
Correction Factor (280 nm) | 0.039 |
Correction Factor (565 nm) | 0.0250 |
Correction Factor (650 nm) | 0.1413 |
Extinction coefficient (cm -1 M -1) | 2750001 |
Excitation (nm) | 757 |
Emission (nm) | 779 |
Quantum yield | 0.121 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Correction Factor (260 nm) 0.044 | Correction Factor (280 nm) 0.039 | Correction Factor (565 nm) 0.0250 | Correction Factor (650 nm) 0.1413 | Extinction coefficient (cm -1 M -1) 2750001 | Excitation (nm) 757 | Emission (nm) 779 | Quantum yield 0.121 |

Figure 1. Overview of the ReadiLink™ xtra Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.
Components
Example protocol
AT A GLANCE
Warm all the components and centrifuge the vials briefly before opening them. Immediately prepare the necessary solutions before starting your conjugation. The following protocol is a recommendation.
PREPARATION OF WORKING SOLUTION
For labeling 50 µg of protein (assuming the target protein concentration is 1 mg/mL), mix 5 µL (10% of the total reaction volume) of Reaction Buffer (Component B) with 50 µL of the target protein solution.
Note: If you have a different protein concentration, adjust the protein volume accordingly to make ~50 µg of protein available for your labeling reaction.
Note: For labeling 100 µg of protein (assuming the target protein concentration is 1 mg/mL), mix 10 µL (10% of the total reaction volume) of Reaction Buffer (Component B) with 100 µL of the target protein solution.
Note: The protein should be dissolved in 1X phosphate buffered saline (PBS), pH 7.2 - 7.4; if the protein is dissolved in glycine buffer, it must be dialyzed against 1X PBS, pH 7.2 - 7.4, or use Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa (cat# UFC501008 from Millipore) to remove free amines or ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation.
Note: Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) with 0.1 to 0.5 % will be labeled well.
Note: A final protein concentration range of 1 - 2 mg/mL is recommended for optimal labeling efficiency, with a significantly reduced conjugation efficiency at less than 1 mg/mL.
SAMPLE EXPERIMENTAL PROTOCOL
Add the protein working solution (Solution A) to ONE vial of labeling dye (Component A), and mix them well by repeatedly pipetting for a few times or vortex the vial for a few seconds.
Note: If labeling 100 µg of protein, use both vials (Component A) of labeling dye by dividing the 100 µg of protein into 2 x 50 µg of protein and reacting each 50 µg of protein with one vial of labeling dye. Then combine both vials for the next step.
Keep the conjugation reaction mixture at room temperature for 30 - 60 minutes.
Note: The conjugation reaction mixture can be rotated or shaken for a longer time if desired.
- Add 5 µL (for 50 µg protein) or 10 µL (for 100 µg protein) which is 10% of the total reaction volume of TQ™-Dyed Quench Buffer (Component C) into the conjugation reaction mixture; mix well.
- Incubate at room temperature for 10 minutes. The labeled protein (antibody) is now ready to use.
The protein conjugate should be stored at > 0.5 mg/mL in the presence of a carrier protein (e.g., 0.1% bovine serum albumin). For longer storage, the protein conjugates could be lyophilized or divided into single-used aliquots and stored at ≤ –20 °C.
Spectrum

Spectral properties
Correction Factor (260 nm) | 0.044 |
Correction Factor (280 nm) | 0.039 |
Correction Factor (565 nm) | 0.0250 |
Correction Factor (650 nm) | 0.1413 |
Extinction coefficient (cm -1 M -1) | 2750001 |
Excitation (nm) | 757 |
Emission (nm) | 779 |
Quantum yield | 0.121 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiLink™ xtra Rapid iFluor® 350 Antibody Labeling Kit *BSA-Compatible* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
ReadiLink™ xtra Rapid iFluor® 488 Antibody Labeling Kit *BSA-Compatible* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
ReadiLink™ xtra Rapid iFluor® 555 Antibody Labeling Kit *BSA-Compatible* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
ReadiLink™ xtra Rapid iFluor® 594 Antibody Labeling Kit *BSA-Compatible* | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
ReadiLink™ xtra Rapid iFluor® 647 Antibody Labeling Kit *BSA-Compatible* | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
Images

References
Authors: Duranti, Claudia and Carraresi, Laura and Sette, Angelica and Stefanini, Matteo and Lottini, Tiziano and Crescioli, Silvia and Crociani, Olivia and Iamele, Luisa and De Jonge, Hugo and Gherardi, Ermanno and Arcangeli, Annarosa
Journal: Oncotarget (2018): 34972-34989
Authors: Rastogi, Shiva K and Anderson, Hailee E and Lamas, Joseph and Barret, Scott and Cantu, Travis and Zauscher, Stefan and Brittain, William J and Betancourt, Tania
Journal: ACS applied materials & interfaces (2018): 30071-30080
Authors: Dong, Lili and Zhou, Hongxin and Zhao, Menglong and Gao, Xinghui and Liu, Yang and Liu, Dongli and Guo, Wei and Hu, Hongwei and Xie, Qian and Fan, Jia and Lin, Jiang and Wu, Weizhong
Journal: Molecular therapy. Nucleic acids (2018): 376-386
Authors: Zhao, Menglong and Dong, Lili and Liu, Zhuang and Yang, Shuohui and Wu, Weizhong and Lin, Jiang
Journal: Quantitative imaging in medicine and surgery (2018): 151-160
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
Authors: Yildiz, Tugba and Gu, Renpeng and Zauscher, Stefan and Betancourt, Tania
Journal: International journal of nanomedicine (2018): 6961-6986
Authors: Seth, Anjali and Lafargue, David and Poirier, Cécile and Badier, Thomas and Delory, Nathalie and Laporte, Alain and Delbos, Jean-Marie and Jeannin, Véronique and Péan, Jean-Manuel and Ménager, Christine
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2017): 25-35
Authors: Saccomano, Mara and Dullin, Christian and Alves, Frauke and Napp, Joanna
Journal: International journal of cancer (2016): 2277-89
Authors: Peterson, Norman C and Wilson, George G and Huang, Qihui and Dimasi, Nazzareno and Sachsenmeier, Kris F
Journal: Comparative medicine (2016): 90-9
Authors: Mahadevan, Kalpana and Patthipati, Venkata Suresh and Han, Sangbum and Swanson, R James and Whelan, Eoin C and Osgood, Christopher and Balasubramanian, Ramjee
Journal: Nanotechnology (2016): 335101
Application notes
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FAQ
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