XFD514 tyramide

XFD514 Tyramide is a green fluorescent dye with an excitation/emission of 518/543 nm that is used for flow cytometry and microscopy applications.

Tyramide Signal Amplification (TSA): Facilitates ultrasensitive detection of low-abundance targets, ideal for IHC and ICC applications
Bright & Stable XFD514 Dye: Delivers intense fluorescence with robust resistance to photobleaching and pH variations (4–10)

Chemical structure for XFD 514 tyramide.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11071	200 Slides	Price

Overview

Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD 514 tyramide contains the Alexa Fluor® 514 fluorophore that can be readily detected with the standard rhodamine 6G filter set (Alexa Fluor® is the trademark of ThermoFisher). XFD 514 tyramide has intense green-yellow fluorescence color.

References

View all 12 references: Citation Explorer

Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Carboxylcytosine in Sections of Zebrafish Embryos.

Authors:

Jessop, Peter and Gering, Martin

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 193-208

Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation.

Authors:

Kuwajima, Masaaki and Ostrovskaya, Olga I and Cao, Guan and Weisberg, Seth A and Harris, Kristen M and Zemelman, Boris V

Journal:

PloS one (2020): e0226797

Intensive Immunofluorescence Staining Methods for Low Expression Protein: Detection of Intestinal Stem Cell Marker LGR5.

Authors:

Yamazaki, Masaki and Kato, Atsuhiko and Zaitsu, Yoko and Watanabe, Takeshi and Iimori, Makoto and Funahashi, Shinichi and Kitao, Hiroyuki and Saeki, Hiroshi and Oki, Eiji and Suzuki, Masami

Journal:

Acta histochemica et cytochemica (2015): 159-64

Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.

Authors:

Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31

Methoxychlor and estradiol induce oxidative stress DNA damage in the mouse ovarian surface epithelium.

Authors:

Symonds, Daniel A and Merchenthaler, Istvan and Flaws, Jodi A

Journal:

Toxicological sciences : an official journal of the Society of Toxicology (2008): 182-7

Physical properties

Molecular weight	938.17
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.31
Correction factor (280 nm)	0.18
Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	518
Emission (nm)	543

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025