XFD594 azide
Product key features
- Ex/Em: 590/618 nm
- Extinction coefficient: 90,000 cm-1M-1
- Reactive Group: Azide
- Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
- Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
- Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
Product description
XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.
The azide derivative of XFD594 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.56 |
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 92000 | 0.56 |
XFD647 Azide | 650 | 671 | 270000 | 0.03 |
XFD350 azide | 343 | 441 | 19000 | 0.19 |
XFD405 azide | 401 | 421 | 35,000 | 0.70 |
XFD430 azide | 432 | 540 | 15,000 | 0.28 |
XFD514 azide | 518 | 543 | 80000 | 0.18 |
XFD546 azide | 561 | 572 | 112000 | 0.12 |
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