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AAT Bioquest

XFD594 azide

Product key features

  • Ex/Em: 590/618 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Reactive Group: Azide
  • Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
  • Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

The azide derivative of XFD594 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.

Spectrum

References

View all 50 references: Citation Explorer
Correlative super-resolution microscopy with deep UV reactivation.
Authors: Prakash, Kirti
Journal: Journal of microscopy (2023)
Amphiphilic Poly(2-oxazoline)s with Glycine-Containing Hydrophobic Blocks Tailored for Panobinostat- and Imatinib-Loaded Micelles.
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Anatomical analyses of collateral prefrontal cortex projections to the basolateral amygdala and the nucleus accumbens core in rats.
Authors: Shih, Cheng-Wei and Chang, Chun-Hui
Journal: Brain structure & function (2023)
A model eye for fluorescent characterization of retinal cultures and tissues.
Authors: Ferraro, G and Gigante, Y and Pitea, M and Mautone, L and Ruocco, G and Di Angelantonio, S and Leonetti, M
Journal: Scientific reports (2023): 10983
Total Flavonoids of Polygala fallax Hemsl Induce Apoptosis of Human Ectopic Endometrial Stromal Cells through PI3K/AKT/Bcl-2 Signaling Pathway.
Authors: Zhong, Chuanmei and Ju, Gongchenhao and Yang, Sufang and Zhao, Xiangpei and Chen, Jixiang and Li, Ning
Journal: Gynecologic and obstetric investigation (2023): 197-213
Page updated on April 25, 2025

Ordering information

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Catalog Number1729
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Additional ordering information

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Physical properties

Molecular weight

993.25

Solvent

DMSO

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
Product Image
Gallery Image 1
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an azide‐functionalized dye and an alkyne‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions and tolerates a wide range of functional groups.