XFD647 Azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1041.11 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (565 nm) | 0.1109 |
| Extinction coefficient (cm -1 M -1) | 270000 |
| Excitation (nm) | 650 |
| Emission (nm) | 671 |
| Quantum yield | 0.331 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Direct upgrades
| iFluor® 647 azide |
Alternative formats
| XFD647 amine |
| XFD647 acid |
| XFD647 Alkyne |
Related products
| Overview |  SDSProtocol |
Upgrade: iFluor® 647 azide
See also: Antibody and Protein Labeling
Molecular weight 1041.11 | Correction Factor (280 nm) 0.03 | Correction Factor (565 nm) 0.1109 | Extinction coefficient (cm -1 M -1) 270000 | Excitation (nm) 650 | Emission (nm) 671 | Quantum yield 0.331 |
XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright deep red fluorescent dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD647 azide is used for conjugation with a biological molecule that contains an alkyne via the click chemistry. It provides a convenient way to install the fluorophore of Alexa Fluor® 647 into a biological molecule that lacks alkyne and azide groups.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 96.051 µL | 480.257 µL | 960.513 µL | 4.803 mL | 9.605 mL |
| 5 mM | 19.21 µL | 96.051 µL | 192.103 µL | 960.513 µL | 1.921 mL |
| 10 mM | 9.605 µL | 48.026 µL | 96.051 µL | 480.257 µL | 960.513 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (565 nm) | 0.1109 |
| Extinction coefficient (cm -1 M -1) | 270000 |
| Excitation (nm) | 650 |
| Emission (nm) | 671 |
| Quantum yield | 0.331 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
| XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 490 | 520 | 0.11 |
| XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin* | 604 | 671 | 0.03 |
| AMCA Azide | 346 | 434 | 0.153 |
| ICG azide | 789 | 814 | 0.076 |
| Cy3B azide | 560 | 571 | 0.069 |
Images
Figure 1. Chemical structure for XFD647 Azide.
References
View all 9 references: Citation Explorer
SMALL MOLECULE IMAGING AGENT FOR MUTANT KRAS G12C.
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)
Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations.
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics.
Authors: Spiciarich, David R and Nolley, Rosalie and Maund, Sophia L and Purcell, Sean C and Herschel, Jason and Iavarone, Anthony T and Peehl, Donna M and Bertozzi, Carolyn R
Journal: Angewandte Chemie (International ed. in English) (2017): 8992-8997
Authors: Spiciarich, David R and Nolley, Rosalie and Maund, Sophia L and Purcell, Sean C and Herschel, Jason and Iavarone, Anthony T and Peehl, Donna M and Bertozzi, Carolyn R
Journal: Angewandte Chemie (International ed. in English) (2017): 8992-8997
Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea.
Authors: Bennett, Kristen and Sadler, Natalie C and Wright, Aaron T and Yeager, Chris and Hyman, Michael R
Journal: Applied and environmental microbiology (2016): 2270-2279
Authors: Bennett, Kristen and Sadler, Natalie C and Wright, Aaron T and Yeager, Chris and Hyman, Michael R
Journal: Applied and environmental microbiology (2016): 2270-2279
Plasmonic nanodiamonds: targeted core-shell type nanoparticles for cancer cell thermoablation.
Authors: Rehor, Ivan and Lee, Karin L and Chen, Kevin and Hajek, Miroslav and Havlik, Jan and Lokajova, Jana and Masat, Milan and Slegerova, Jitka and Shukla, Sourabh and Heidari, Hamed and Bals, Sara and Steinmetz, Nicole F and Cigler, Petr
Journal: Advanced healthcare materials (2015): 460-8
Authors: Rehor, Ivan and Lee, Karin L and Chen, Kevin and Hajek, Miroslav and Havlik, Jan and Lokajova, Jana and Masat, Milan and Slegerova, Jitka and Shukla, Sourabh and Heidari, Hamed and Bals, Sara and Steinmetz, Nicole F and Cigler, Petr
Journal: Advanced healthcare materials (2015): 460-8
Labeling proteins on live mammalian cells using click chemistry.
Authors: Nikić, Ivana and Kang, Jun Hee and Girona, Gemma Estrada and Aramburu, Iker Valle and Lemke, Edward A
Journal: Nature protocols (2015): 780-91
Authors: Nikić, Ivana and Kang, Jun Hee and Girona, Gemma Estrada and Aramburu, Iker Valle and Lemke, Edward A
Journal: Nature protocols (2015): 780-91
Double click: dual functionalized polymeric micelles with antibodies and peptides.
Authors: Chan, Dianna P Y and Owen, Shawn C and Shoichet, Molly S
Journal: Bioconjugate chemistry (2013): 105-13
Authors: Chan, Dianna P Y and Owen, Shawn C and Shoichet, Molly S
Journal: Bioconjugate chemistry (2013): 105-13
Synthesis of a library of fucosylated glycoclusters and determination of their binding toward Pseudomonas aeruginosa lectin B (PA-IIL) using a DNA-based carbohydrate microarray.
Authors: Gerland, Béatrice and Goudot, Alice and Pourceau, Gwladys and Meyer, Albert and Dugas, Vincent and Cecioni, Samy and Vidal, Sébastien and Souteyrand, Eliane and Vasseur, Jean-Jacques and Chevolot, Yann and Morvan, François
Journal: Bioconjugate chemistry (2012): 1534-47
Authors: Gerland, Béatrice and Goudot, Alice and Pourceau, Gwladys and Meyer, Albert and Dugas, Vincent and Cecioni, Samy and Vidal, Sébastien and Souteyrand, Eliane and Vasseur, Jean-Jacques and Chevolot, Yann and Morvan, François
Journal: Bioconjugate chemistry (2012): 1534-47
Intravital imaging of human prostate cancer using viral nanoparticles targeted to gastrin-releasing Peptide receptors.
Authors: Steinmetz, Nicole F and Ablack, Amber L and Hickey, Jennifer L and Ablack, Jailal and Manocha, Bhavik and Mymryk, Joe S and Luyt, Leonard G and Lewis, John D
Journal: Small (Weinheim an der Bergstrasse, Germany) (2011): 1664-72
Authors: Steinmetz, Nicole F and Ablack, Amber L and Hickey, Jennifer L and Ablack, Jailal and Manocha, Bhavik and Mymryk, Joe S and Luyt, Leonard G and Lewis, John D
Journal: Small (Weinheim an der Bergstrasse, Germany) (2011): 1664-72