XFD647 Azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1041.11
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.03
Correction Factor (565 nm)	0.1109
Extinction coefficient (cm ^-1 M ^-1)	270000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Alternative formats

Overview SDS Protocol

Upgrade: iFluor® 647 azide

Molecular weight

1041.11

Correction Factor (280 nm)

0.03

Correction Factor (565 nm)

0.1109

Extinction coefficient (cm ^-1 M ^-1)

270000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.33¹

XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright deep red fluorescent dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD647 azide is used for conjugation with a biological molecule that contains an alkyne via the click chemistry. It provides a convenient way to install the fluorophore of Alexa Fluor® 647 into a biological molecule that lacks alkyne and azide groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.051 µL	480.257 µL	960.513 µL	4.803 mL	9.605 mL
5 mM	19.21 µL	96.051 µL	192.103 µL	960.513 µL	1.921 mL
10 mM	9.605 µL	48.026 µL	96.051 µL	480.257 µL	960.513 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.03
Correction Factor (565 nm)	0.1109
Extinction coefficient (cm ^-1 M ^-1)	270000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Images

Figure 1. Chemical structure for XFD647 Azide.

References

View all 9 references: Citation Explorer

SMALL MOLECULE IMAGING AGENT FOR MUTANT KRAS G12C.
Authors: Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph
Journal: Advanced therapeutics (2021)

Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations.
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515

Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomics.
Authors: Spiciarich, David R and Nolley, Rosalie and Maund, Sophia L and Purcell, Sean C and Herschel, Jason and Iavarone, Anthony T and Peehl, Donna M and Bertozzi, Carolyn R
Journal: Angewandte Chemie (International ed. in English) (2017): 8992-8997

Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea.
Authors: Bennett, Kristen and Sadler, Natalie C and Wright, Aaron T and Yeager, Chris and Hyman, Michael R
Journal: Applied and environmental microbiology (2016): 2270-2279

Plasmonic nanodiamonds: targeted core-shell type nanoparticles for cancer cell thermoablation.
Authors: Rehor, Ivan and Lee, Karin L and Chen, Kevin and Hajek, Miroslav and Havlik, Jan and Lokajova, Jana and Masat, Milan and Slegerova, Jitka and Shukla, Sourabh and Heidari, Hamed and Bals, Sara and Steinmetz, Nicole F and Cigler, Petr
Journal: Advanced healthcare materials (2015): 460-8

Labeling proteins on live mammalian cells using click chemistry.
Authors: Nikić, Ivana and Kang, Jun Hee and Girona, Gemma Estrada and Aramburu, Iker Valle and Lemke, Edward A
Journal: Nature protocols (2015): 780-91

Double click: dual functionalized polymeric micelles with antibodies and peptides.
Authors: Chan, Dianna P Y and Owen, Shawn C and Shoichet, Molly S
Journal: Bioconjugate chemistry (2013): 105-13

Synthesis of a library of fucosylated glycoclusters and determination of their binding toward Pseudomonas aeruginosa lectin B (PA-IIL) using a DNA-based carbohydrate microarray.
Authors: Gerland, Béatrice and Goudot, Alice and Pourceau, Gwladys and Meyer, Albert and Dugas, Vincent and Cecioni, Samy and Vidal, Sébastien and Souteyrand, Eliane and Vasseur, Jean-Jacques and Chevolot, Yann and Morvan, François
Journal: Bioconjugate chemistry (2012): 1534-47

Intravital imaging of human prostate cancer using viral nanoparticles targeted to gastrin-releasing Peptide receptors.
Authors: Steinmetz, Nicole F and Ablack, Amber L and Hickey, Jennifer L and Ablack, Jailal and Manocha, Bhavik and Mymryk, Joe S and Luyt, Leonard G and Lewis, John D
Journal: Small (Weinheim an der Bergstrasse, Germany) (2011): 1664-72