FastClick™ 6-TAMRA Alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 738.89 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ 6-TAMRA Azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 738.89 |
FastClick™ 6-TAMRA Alkyne contains both the moiety of FastClick (for assisting click efficiency) and 6-TAMRA fluorophore (as the fluorescence tag) for developing 6-TAMRA-based fluorescent probes. It readily reacts with an azido-modified biomolecule under extremely mild conditions. 6-TAMRA is one of the most common orange-red fluorophores used for labeling biomolecules, in particular, for labeling oligonucleotides. 6-TAMRA conjugates are widely used in fluorescence-based nucleic acid detections such PCR and FISH etc. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ 6-TAMRA Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 135.338 µL | 676.691 µL | 1.353 mL | 6.767 mL | 13.534 mL |
5 mM | 27.068 µL | 135.338 µL | 270.676 µL | 1.353 mL | 2.707 mL |
10 mM | 13.534 µL | 67.669 µL | 135.338 µL | 676.691 µL | 1.353 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
References
View all 50 references: Citation Explorer
Mechanochromic and Solvomechanochromic Fluorescent Photonic Crystals for Dual-Mode Modulating Fluorescence and Multilevel Anticounterfeiting.
Authors: Liu, Junfu and Ma, Dekun and Qi, Chenze and Yang, Dongpeng and Huang, Shaoming
Journal: ACS applied materials & interfaces (2024): 2740-2750
Authors: Liu, Junfu and Ma, Dekun and Qi, Chenze and Yang, Dongpeng and Huang, Shaoming
Journal: ACS applied materials & interfaces (2024): 2740-2750
Efforts toward PET-Activatable Red-Shifted Silicon Rhodamines and Silicon Pyronine Dyes.
Authors: Kramer, Carsten Sven and Kanagasundaram, Thines and Matthias, Jessica and Kopka, Klaus
Journal: Pharmaceuticals (Basel, Switzerland) (2023)
Authors: Kramer, Carsten Sven and Kanagasundaram, Thines and Matthias, Jessica and Kopka, Klaus
Journal: Pharmaceuticals (Basel, Switzerland) (2023)
Synthesis and Optoelectronic Features of Rhodamine-Triazole Dyads as Metallochromic Probes for Copper-Selective Chemosensing.
Authors: Vadivelu, Murugan and Raheem, Abbasriyaludeen Abdul and Shankar, Bhaskaran and Karthikeyan, Kesavan and Praveen, Chandrasekar
Journal: Chemistry, an Asian journal (2023): e202300098
Authors: Vadivelu, Murugan and Raheem, Abbasriyaludeen Abdul and Shankar, Bhaskaran and Karthikeyan, Kesavan and Praveen, Chandrasekar
Journal: Chemistry, an Asian journal (2023): e202300098
Dual-mode colorimetric/fluorescent chemosensor for Cu2+/Zn2+ and fingerprint imaging based on rhodamine ethylenediamine bis(triazolyl silsesquioxane).
Authors: Dare, Enock O and Akinhanmi, Temilade F and Aremu, J A and Adetunji, Olayide R and Bamgbose, Janet T and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Pérez-Ruiz, Raúl and Bonardd, Sebastian and Díaz Díaz, David
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2023): 1527-1541
Authors: Dare, Enock O and Akinhanmi, Temilade F and Aremu, J A and Adetunji, Olayide R and Bamgbose, Janet T and Vendrell-Criado, Victoria and Jiménez, M Consuelo and Pérez-Ruiz, Raúl and Bonardd, Sebastian and Díaz Díaz, David
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2023): 1527-1541
New Carboxytriazolyl Amphiphilic Derivatives of Calix[4]arenes: Aggregation and Use in CuAAC Catalysis.
Authors: Mironova, Diana and Bogdanov, Ilshat and Akhatova, Aliya and Sultanova, Elza and Garipova, Ramilya and Khannanov, Artur and Burilov, Vladimir and Solovieva, Svetlana and Antipin, Igor
Journal: International journal of molecular sciences (2023)
Authors: Mironova, Diana and Bogdanov, Ilshat and Akhatova, Aliya and Sultanova, Elza and Garipova, Ramilya and Khannanov, Artur and Burilov, Vladimir and Solovieva, Svetlana and Antipin, Igor
Journal: International journal of molecular sciences (2023)
A Rhodamine-coumarin Triazole Conjugate as a Fluorescent Chemodosimeter for Cu(II) Detection and its Application in Live Cell Bioimaging.
Authors: Wechakorn, Kanokorn and Pitsanuwong, Chariwat and Kanjanasirirat, Phongthon and Pewkliang, Yongyut and Borwornpinyo, Suparerk and Kongsaeree, Palangpon
Journal: Journal of fluorescence (2023)
Authors: Wechakorn, Kanokorn and Pitsanuwong, Chariwat and Kanjanasirirat, Phongthon and Pewkliang, Yongyut and Borwornpinyo, Suparerk and Kongsaeree, Palangpon
Journal: Journal of fluorescence (2023)
Divergent Synthesis of Ultrabright and Dendritic Xanthenes for Enhanced Click-Chemistry-Based Bioimaging.
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Quantitative and biosafe modification of bifunctional groups onto carbon dots by click chemistry.
Authors: He, Qian and Wu, Zewen and Li, Jingxuan and Li, Ruijiao and Zhang, Liyun and Liu, Yaodong
Journal: Journal of materials chemistry. B (2023): 5094-5100
Authors: He, Qian and Wu, Zewen and Li, Jingxuan and Li, Ruijiao and Zhang, Liyun and Liu, Yaodong
Journal: Journal of materials chemistry. B (2023): 5094-5100
Bio-orthogonal Toolbox for Monitoring Nitric Oxide in Targeted Organelles of Live Cells and Zebrafishes.
Authors: Zhou, Lin and Liu, Chuanhao and Zheng, Ying and Huang, Zhenlong and Zhang, Xinfu and Xiao, Yi
Journal: Analytical chemistry (2022): 15678-15685
Authors: Zhou, Lin and Liu, Chuanhao and Zheng, Ying and Huang, Zhenlong and Zhang, Xinfu and Xiao, Yi
Journal: Analytical chemistry (2022): 15678-15685
Cross-linked polymer microparticles with tunable surface properties by the combination of suspension free radical copolymerization and Click chemistry.
Authors: Moratille, Yoanh and Arshad, Muhammad and Cohen, Celine and Maali, Abdelhamid and Lemaire, Elisabeth and Sintes-Zydowicz, Nathalie and Drockenmuller, Eric
Journal: Journal of colloid and interface science (2022): 1687-1698
Authors: Moratille, Yoanh and Arshad, Muhammad and Cohen, Celine and Maali, Abdelhamid and Lemaire, Elisabeth and Sintes-Zydowicz, Nathalie and Drockenmuller, Eric
Journal: Journal of colloid and interface science (2022): 1687-1698