FastClick™ Cy5 Alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 965.24 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ Cy5 Azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 965.24 | Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
FastClick™ Cy5 Alkyne contains both the moiety of FastClick (for assisting click efficiency) and Cy5 fluorophore (as the fluorescence tag) for developing Cy5-based fluorescent probes. It readily reacts with an azido-modified biomolecule under extremely mild conditions. Cy5 is a common deep red fluorophore in combination with Cy3 (orange-red fluorescence) for dual color detections. Cy5 conjugates are widely used in fluorescence imaging and flow cytometric applications. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ Cy5 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 103.601 µL | 518.006 µL | 1.036 mL | 5.18 mL | 10.36 mL |
5 mM | 20.72 µL | 103.601 µL | 207.202 µL | 1.036 mL | 2.072 mL |
10 mM | 10.36 µL | 51.801 µL | 103.601 µL | 518.006 µL | 1.036 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
FastClick™ Cy3 Alkyne | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
FastClick™ Cy7 Alkyne | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
FastClick™ XFD350 Alkyne | 343 | 441 | 19000 | - | 0.25 | 0.19 |
FastClick™ XFD488 Alkyne | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
FastClick™ XFD555 Alkyne | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
FastClick™ XFD647 Alkyne | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
FastClick™ XFD750 Alkyne | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Images
Figure 1. The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne (Red Bar) does not effectively react with the coumarin azide substrate.
References
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Engineering Neurotoxin-Functionalized Exosomes for Targeted Delivery to the Peripheral Nervous System.
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Journal: Pharmaceutics (2024)
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Journal: Pharmaceutics (2024)
Fluorescent Properties of Cyanine Dyes As a Matter of the Environment.
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Journal: Journal of fluorescence (2023)
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Journal: Journal of the American Chemical Society (2023)
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Journal: Journal of the American Chemical Society (2023)
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Authors: Zhang, Xiaoqin and Wang, Xiaoming and Li, Zhiqian and Du, Jun and Xiao, Xueyang and Pan, Dayi and Zhang, Hu and Tian, Xiaohe and Gong, Qiyong and Gu, Zhongwei and Luo, Kui
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Authors: Zhang, Xiaoqin and Wang, Xiaoming and Li, Zhiqian and Du, Jun and Xiao, Xueyang and Pan, Dayi and Zhang, Hu and Tian, Xiaohe and Gong, Qiyong and Gu, Zhongwei and Luo, Kui
Journal: Nanoscale (2023): 809-819
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Journal: Talanta (2023): 124972
Efficient Labeling of Nanocellulose for High-Resolution Fluorescence Microscopy Applications.
Authors: Babi, Mouhanad and Fatona, Ayodele and Li, Xiang and Cerson, Christine and Jarvis, Victoria M and Abitbol, Tiffany and Moran-Mirabal, Jose M
Journal: Biomacromolecules (2022): 1981-1994
Authors: Babi, Mouhanad and Fatona, Ayodele and Li, Xiang and Cerson, Christine and Jarvis, Victoria M and Abitbol, Tiffany and Moran-Mirabal, Jose M
Journal: Biomacromolecules (2022): 1981-1994
Click Chemistry-Enabled Conjugation Strategy for Producing Dibenzodiazepinone-Type Fluorescent Probes To Target M2 Acetylcholine Receptors.
Authors: Yang, Hongrong and Micovic, Nicholas and Monaghan, James R and Clark, Heather A
Journal: Bioconjugate chemistry (2022): 2223-2233
Authors: Yang, Hongrong and Micovic, Nicholas and Monaghan, James R and Clark, Heather A
Journal: Bioconjugate chemistry (2022): 2223-2233
Multi-antitumor therapy and synchronous imaging monitoring based on exosome.
Authors: Qian, Ruijie and Jing, Boping and Jiang, Dawei and Gai, Yongkang and Zhu, Ziyang and Huang, Xiaojuan and Gao, Yu and Lan, Xiaoli and An, Rui
Journal: European journal of nuclear medicine and molecular imaging (2022): 2668-2681
Authors: Qian, Ruijie and Jing, Boping and Jiang, Dawei and Gai, Yongkang and Zhu, Ziyang and Huang, Xiaojuan and Gao, Yu and Lan, Xiaoli and An, Rui
Journal: European journal of nuclear medicine and molecular imaging (2022): 2668-2681
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Journal: Nano letters (2022): 151-156
Authors: Luo, Rui and Ou, Chunqing and Li, Xinchao and Wang, Yanfang and Du, Wei and Liang, Gaolin and Gong, Changyang
Journal: Nano letters (2022): 151-156
Chemical Synthesis of a Functional Fluorescent-Tagged α-Bungarotoxin.
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Journal: Toxins (2022)
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Journal: Toxins (2022)