FastClick™ XFD750 Alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1307.49 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 240000 |
Excitation (nm) | 752 |
Emission (nm) | 776 |
Quantum yield | 0.121 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ XFD750 Azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 1307.49 | Correction Factor (260 nm) 0.00 | Correction Factor (280 nm) 0.04 | Extinction coefficient (cm -1 M -1) 240000 | Excitation (nm) 752 | Emission (nm) 776 | Quantum yield 0.121 |
FastClick™ XFD750 Alkyne contains both the CAG moiety of FastClick (for assisting click efficiency) and Alexa Fluor® 750 fluorophore (as the fluorescence tag) for developing Alexa Fluor® 750-based fluorescent probes. It readily reacts with an azido-containing biomolecule under extremely mild conditions. Alexa Fluor® 750 is a commonly used near infrared (NIR) fluorophore for labeling proteins, nucleic acids, or other biomolecules. It has moderate photostability and excitation that matches the common 750 nm laser line. Its conjugates are widely used for in vivo imaging and flow cytometry applications in combination with the widely available Cy7 filter set. It is a water-soluble Cy75 derivative that has pH-insensitive fluorescence from pH 4 to pH 10. Alexa Fluor® 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM. It is also used in super-resolution microscopy (SRM) applications. Alexa Fluor® is a trademark of ThermoFisher Scientific. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ XFD750 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 76.482 µL | 382.412 µL | 764.824 µL | 3.824 mL | 7.648 mL |
5 mM | 15.296 µL | 76.482 µL | 152.965 µL | 764.824 µL | 1.53 mL |
10 mM | 7.648 µL | 38.241 µL | 76.482 µL | 382.412 µL | 764.824 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 240000 |
Excitation (nm) | 752 |
Emission (nm) | 776 |
Quantum yield | 0.121 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
FastClick™ Cy3 Alkyne | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
FastClick™ Cy5 Alkyne | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
FastClick™ Cy7 Alkyne | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
FastClick™ XFD350 Alkyne | 343 | 441 | 19000 | - | 0.25 | 0.19 |
FastClick™ XFD488 Alkyne | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
FastClick™ XFD555 Alkyne | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
FastClick™ XFD647 Alkyne | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
Images
Figure 1. The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
Figure 2. FastClick™ XFD750 Alkyne contains both the CAG moiety of FastClick (for assisting click efficiency) and Alexa Fluor® 750 fluorophore (as the fluorescence tag) for developing Alexa Fluor® 750-based fluorescent probes. It readily reacts with an azido-containing biomolecule under extremely mild conditions.
References
View all 5 references: Citation Explorer
Targeting lung cancer with clinically relevant EGFR mutations using anti-EGFR RNA aptamer.
Authors: Thomas, Brian J and Guldenpfennig, Caitlyn and Guan, Yue and Winkler, Calvin and Beecher, Margaret and Beedy, Michaela and Berendzen, Ashley F and Ma, Lixin and Daniels, Mark A and Burke, Donald H and Porciani, David
Journal: Molecular therapy. Nucleic acids (2023): 102046
Authors: Thomas, Brian J and Guldenpfennig, Caitlyn and Guan, Yue and Winkler, Calvin and Beecher, Margaret and Beedy, Michaela and Berendzen, Ashley F and Ma, Lixin and Daniels, Mark A and Burke, Donald H and Porciani, David
Journal: Molecular therapy. Nucleic acids (2023): 102046
Circulating Regulatory T Cell Subsets in Patients with Sarcoidosis.
Authors: Kudryavtsev, Igor and Zinchenko, Yulia and Starshinova, Anna and Serebriakova, Maria and Malkova, Anna and Akisheva, Tatiana and Kudlay, Dmitriy and Glushkova, Anzhela and Yablonskiy, Piotr and Shoenfeld, Yehuda
Journal: Diagnostics (Basel, Switzerland) (2023)
Authors: Kudryavtsev, Igor and Zinchenko, Yulia and Starshinova, Anna and Serebriakova, Maria and Malkova, Anna and Akisheva, Tatiana and Kudlay, Dmitriy and Glushkova, Anzhela and Yablonskiy, Piotr and Shoenfeld, Yehuda
Journal: Diagnostics (Basel, Switzerland) (2023)
iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility.
Authors: Jensen, Holly A and Kim, Jeong
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)
Authors: Jensen, Holly A and Kim, Jeong
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)
[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates.
Authors: Berlier, Judith E and Rothe, Anca and Buller, Gayle and Bradford, Jolene and Gray, Diane R and Filanoski, Brian J and Telford, William G and Yue, Stephen and Liu, Jixiang and Cheung, Ching-Ying and Chang, Wesley and Hirsch, James D and Beechem, Joseph M and Haugland, Rosaria P and Haugland, Richard P
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2003): 1699-712
Authors: Berlier, Judith E and Rothe, Anca and Buller, Gayle and Bradford, Jolene and Gray, Diane R and Filanoski, Brian J and Telford, William G and Yue, Stephen and Liu, Jixiang and Cheung, Ching-Ying and Chang, Wesley and Hirsch, James D and Beechem, Joseph M and Haugland, Rosaria P and Haugland, Richard P
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2003): 1699-712