FastClick™ Cy5 Azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 869.07 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ Cy5 Alkyne |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 869.07 | Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
FastClick™ Cy5 Azide contains both the CAG moiety of FastClick (for assisting click efficiency) and Cy5 fluorophore (as the fluorescence tag) for developing Cy5-based fluorescent probes. Cy5 is one of the most widely used red fluorophores. It has the identical fluorescence spectra to Alexa Fluor 647. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ Cy5 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 115.066 µL | 575.328 µL | 1.151 mL | 5.753 mL | 11.507 mL |
5 mM | 23.013 µL | 115.066 µL | 230.131 µL | 1.151 mL | 2.301 mL |
10 mM | 11.507 µL | 57.533 µL | 115.066 µL | 575.328 µL | 1.151 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
FastClick™ Cy3 Azide | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
FastClick™ Cy7 Azide | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
FastClick™ XFD350 Azide | 343 | 441 | 19000 | - | 0.25 | 0.19 |
FastClick™ XFD488 Azide | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
FastClick™ XFD555 Azide | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
FastClick™ XFD647 Azide | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
FastClick™ XFD750 Azide | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Images
Figure 1. FastClick™ Cy5 Azide contains both the moiety of FastClick (for assisting click efficiency) and Cy5 fluorophore (as the fluorescence tag) for developing Cy5-based fluorescent probes. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction.
Figure 2. The reaction (Green Bar) of FastClick Cy5 Azide with coumarin alkyne occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 azide does not effectively react with the coumarin alkyne substrate.
References
View all 2 references: Citation Explorer
Development of a general methodology for labelling peptide-morpholino oligonucleotide conjugates using alkyne-azide click chemistry.
Authors: Shabanpoor, Fazel and Gait, Michael J
Journal: Chemical communications (Cambridge, England) (2013): 10260-2
Authors: Shabanpoor, Fazel and Gait, Michael J
Journal: Chemical communications (Cambridge, England) (2013): 10260-2
Two-strain, cell-selective protein labeling in mixed bacterial cultures.
Authors: Truong, Frank and Yoo, Tae Hyeon and Lampo, Thomas J and Tirrell, David A
Journal: Journal of the American Chemical Society (2012): 8551-6
Authors: Truong, Frank and Yoo, Tae Hyeon and Lampo, Thomas J and Tirrell, David A
Journal: Journal of the American Chemical Society (2012): 8551-6