Name: Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 12602
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Neuraminidase Assay Kit

Blue Fluorescence

Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence* is a fluorimetric assay kit for detection of neuraminidase activity in cells and biological samples.

Detection Principle: Uses a non-fluorescent neuraminidase substrate that becomes strongly fluorescent upon enzymatic cleavage.
Sensitivity: Detects neuraminidase activity down to 0.3 mU/mL in a 100 µL assay.
Workflow: Compatible with 96- or 384-well microplates and can be easily adapted to automation without a separation step.
Instrument Compatibility: Fluorescence detection at Ex/Em 320/460 nm using solid black microplates.

Neuraminidase dose response was measured in a 96-well black plate with Amplite® Fluorimetric Neuraminidase Assay Kit using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
12602	200 Tests	Price

Overview

Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid. The most commonly known neuraminidase is the viral neuraminidase. The cleavage of linkage between sialic acid and adjacent sugar residue permits the transport of the virus through mucin and destroys the haemagglutinin receptor on the host cell, thus allowing elution of progeny virus particles from infected cells. Neuraminidase promotes influenza virus release from infected cells and facilitates virus spread within the respiratory tract. Thus, it is an important target for influenza drug development. The detection of neuraminidase and screening its inhibitors is one of the essential tasks for investigating biological processes and prevention of influenza infection. There are a few assay kits available for detecting neuraminidase, but all the commercial available kits are tedious to use. Our Amplite® Fluorimetric Neuraminidase Assay Kit provides a sensitiveand robust fluorimetric assay to detect neuraminidase that exists either in cells or biological samples. The non-fluorescent neuraminidase substrate becomes strongly fluorescent upon neuraminidase cleavage. The kit can detect as little as 0.3 mU/mL neuraminidase in a 100 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The signal can be easily read by a fluorescence microplate reader.

References

View all 50 references: Citation Explorer

Rapid screening, identification, and purification of neuraminidase inhibitors from Lithospermum erythrorhizon Sieb.et Zucc. by ultrafiltration with HPLC-ESI-TOF-MS combined with semipreparative HPLC

Authors:

Zhang M, Zhao H, Zhao Z, Yan H, Lv R, Cui L, Yuan J, Wang D, Geng Y, Liu D, Wang X.

Journal:

J Sep Sci (2016): 2097

Neuraminidase inhibiting antibody responses in pigs differ between influenza A virus N2 lineages and by vaccine type

Authors:

S, undefined and bulte MR, Gauger PC, Kitikoon P, Chen H, Perez DR, Roth JA, Vincent AL.

Journal:

Vaccine (2016): 3773

Development of a surface plasmon resonance assay to measure the binding affinity of wild-type influenza neuraminidase and its H274Y mutant to the antiviral drug zanamivir

Authors:

Somasundaram B, Fee CJ, Fredericks R, Watson AJ, Fairbanks AJ.

Journal:

J Mol Recognit (2015): 87

Susceptibility of human influenza A (H3N2) viruses to neuraminidase inhibitors isolated during 2011-2012 in China

Authors:

Huang W, Tan M, Zhao X, Cheng Y, Li X, Guo J, Wei H, Xiao N, Wang Z, Wang D, Shu Y.

Journal:

Zhonghua Yu Fang Yi Xue Za Zhi (2015): 481

Neuraminidase-inhibition assay for the identification of influenza A virus neuraminidase virus subtype or neuraminidase antibody specificity

Authors:

Pedersen JC., undefined

Journal:

Methods Mol Biol (2014): 27

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	320 nm
Emission	460 nm
Cutoff	420 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: FluLite™ Blue	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Neuraminidase Standard	1 vial (0.1 U)