APC-iFluor® 750 Tandem
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Solvent | Water |
Spectral properties
Absorbance (nm) | 754 |
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 776 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
APC-iFluor® 750-streptavidin conjugate |
Overview | SDSProtocol |
Absorbance (nm) 754 | Extinction coefficient (cm -1 M -1) 700000 | Excitation (nm) 651 | Emission (nm) 776 |
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC- iFluor® 750 tandem is an excellent replacement to the commonly used APC-Cy7 with improved FRET efficiency and signal. Its primary absorption peak is at 651 nm with emission peak at ~780 nm. AAT Bioquest also offers its preactivated APC- iFluor® 750 tandem to facilitate the APC- iFluor® 750 tandem tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC- iFluor® 750 is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC- iFluor® 750 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that must be regenerated by the reduction of antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 754 |
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 776 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
APC-iFluor® 700 Tandem | 651 | 710 | 700000 |
APC-iFluor® 800 Tandem | 651 | 819 | 700000 |
APC-iFluor® 710 Tandem | 651 | 747 | 700000 |
APC-iFluor®A7 Tandem | 651 | 782 | 700000 |
APC-iFluor® 720 Tandem | 651 | 748 | 700000 |
APC-iFluor® 740 Tandem | 651 | 764 | 700000 |
APC-iFluor® 770 Tandem | 780 | 800 | 700000 |
APC-iFluor® 780 Tandem | 787 | 812 | 700000 |
PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
Images
References
View all 17 references: Citation Explorer
CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080
Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.
Authors: Elezov, D S and Kudryavtsev, I V and Arsent'ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42
Authors: Elezov, D S and Kudryavtsev, I V and Arsent'ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42
A flow cytometric method for the analysis of macrophages in the vascular wall.
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43
Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77
The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86
Measurement conditions for flow cytometry analyses of cell lines from urological carcinomas.
Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86
Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood.
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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Buccutite™ Bioconjugation Technology
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Buccutite™ Bioconjugation Technology
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
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