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AAT Bioquest

APC-iFluor® 750 Tandem

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC- iFluor® 750 tandem is an excellent replacement to the commonly used APC-Cy7 with improved FRET efficiency and signal. Its primary absorption peak is at 651 nm with emission peak at ~780 nm. AAT Bioquest also offers its preactivated APC- iFluor® 750 tandem to facilitate the APC- iFluor® 750 tandem tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC- iFluor® 750 is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC- iFluor® 750 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that must be regenerated by the reduction of antibodies.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
APC-iFluor® 700 Tandem651710700000
APC-iFluor® 800 Tandem651819700000
APC-iFluor® 710 Tandem651747700000
APC-iFluor®A7 Tandem651782700000
APC-iFluor® 720 Tandem651748700000
APC-iFluor® 740 Tandem651764700000
APC-iFluor® 770 Tandem780800700000
APC-iFluor® 780 Tandem787812700000
PE-iFluor® 750 Tandem5657781960000

References

View all 17 references: Citation Explorer
CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080
Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.
Authors: Elezov, D S and Kudryavtsev, I V and Arsent'ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42
A flow cytometric method for the analysis of macrophages in the vascular wall.
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43
Page updated on December 6, 2024

Ordering information

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Catalog Number2626
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Additional ordering information

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Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)

754

Extinction coefficient (cm -1 M -1)

700000

Excitation (nm)

651

Emission (nm)

776

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Flow cytometry analysis of whole blood cells stained with APC/iFluor® 750 anti-human CD4 antibody (Clone: SK3). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 750 specific R7-A channel.
Flow cytometry analysis of whole blood cells stained with APC/iFluor® 750 anti-human CD4 antibody (Clone: SK3). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 750 specific R7-A channel.
Flow cytometry analysis of whole blood cells stained with APC/iFluor® 750 anti-human CD4 antibody (Clone: SK3). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 750 specific R7-A channel.
Flow cytometry analysis of PBMC stained with APC-iFluor® 750 anti-human CD3 *UCHT1* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 750 specific R7-A channel.