APC-iFluor® 770 Tandem

APC-iFluor® 770 Tandem is an APC-based near-infrared fluorescent tandem dye designed for multicolor and spectral flow cytometry applications.

APC-based Tandem Dye: Uses allophycocyanin as the donor fluorophore with iFluor® 770 as the acceptor dye.
Red Laser Excitation: Primary excitation at 651 nm supports red laser-compatible flow cytometry.
Near-infrared Emission: Provides fluorescence emission at 803 nm for multicolor panel design.
Spectral Flow Compatibility: Validated using the Cytek Aurora spectral flow cytometer.
Applications: Suitable for flow cytometry and fluorescence imaging.

Flow cytometry analysis of whole blood stained with APC-iFluor® 770 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 770 specific R8-A channel.

Datasheet

SDS

Catalog	Size	Price	Quantity
2638	1 mg	Price

Overview

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-iFluor® 770 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. Its primary absorption peak is at 651 nm with emission peak at ~803 nm. It is one of the brightest NIR fluorophores that can be used with flow cytometry and fluorescence imaging applications.

References

View all 2 references: Citation Explorer

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.

Authors:

Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

Cy7PE and Cy7APC: bright new probes for immunofluorescence.

Authors:

Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A

Journal:

Cytometry (1996): 191-7

Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	803

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on October 25, 2026