Cal-590™-Dextran Conjugate MW 3,000

ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-590® AM (red) or Rhod-2 AM (blue)under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a 96-well black wall/clear bottom Costar plate. 100 µL of 5 µg/mL Cal-590® AM or Rhod-2 AM with 2.5 mM probenecid was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 µL/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.

The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	~4000
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	78000
Excitation (nm)	563
Emission (nm)	584
Quantum yield	0.62¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Cal-590™-Dextran Conjugate *MW 10,000*

Overview SDS Protocol

Molecular weight

~4000

Extinction coefficient (cm ^-1 M ^-1)

78000

Excitation (nm)

563

Emission (nm)

584

Quantum yield

0.62¹

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.

Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	78000
Excitation (nm)	563
Emission (nm)	584
Quantum yield	0.62¹

Product Family

Name	Excitation (nm)	Emission (nm)
Cal-590L® Dextran Conjugate MW 10,000	569	590
Cal-630™-Dextran Conjugate MW 3,000	609	626
Cal-630™-Dextran Conjugate MW 10,000	609	626
Cal-520®-Dextran Conjugate MW 3,000	493	515
Cal-520®-Dextran Conjugate MW 10,000	493	515
Cal-670™-Dextran Conjugate MW 3,000	667	680
Cal-670™-Dextran Conjugate MW 10,000	667	680
Cal-770™-Dextran Conjugate MW 3,000	758	783
Cal-770™-Dextran Conjugate MW 10,000	758	783

Images

Figure 1. ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-590® AM (red) or Rhod-2 AM (blue)under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a 96-well black wall/clear bottom Costar plate. 100 µL of 5 µg/mL Cal-590® AM or Rhod-2 AM with 2.5 mM probenecid was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 µL/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.

Figure 2. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.

Citations

View all 6 citations: Citation Explorer

Zebrafish oxytocin neurons drive nocifensive behavior via brainstem premotor targets
Authors: Wee, Caroline L and Nikitchenko, Maxim and Wang, Wei-Chun and Luks-Morgan, Sasha J and Song, Erin and Gagnon, James A and Randlett, Owen and Bianco, Isaac H and Lacoste, Alix MB and Glushenkova, Elena and others,
Journal: Nature neuroscience (2019): 1

Behavioral role of the reciprocal inhibition between a pair of Mauthner cells during fast escapes in zebrafish
Authors: Shimazaki, Takashi and Tanimoto, Masashi and Oda, Yoichi and Higashijima, Shin-ichi
Journal: Journal of Neuroscience (2018): 1964--18

Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505

α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194

In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)

Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382

References

View all 32 references: Citation Explorer

High Affinity Mannotetraose as an Alternative to Dextran in ConA Based Fluorescent Affinity Glucose Assay Due to Improved FRET Efficiency
Authors: Locke, A. K.; Cummins, B. M.; Cote, G. L.
Journal: ACS Sens (2016): 584-590

Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite
Authors: Jin, X.; Zhang, M.; Gou, G.; Ren, J.
Journal: J Pharm Sci (2016): 1751-61

The effect of the size of fluorescent dextran on its endocytic pathway
Authors: Li, L.; Wan, T.; Wan, M.; Liu, B.; Cheng, R.; Zhang, R.
Journal: Cell Biol Int (2015): 531-9

Characterization of biomimetic calcium phosphate labeled with fluorescent dextran for quantification of osteoclastic activity
Authors: Maria, S. M.; Prukner, C.; Sheikh, Z.; Muller, F. A.; Komarova, S. V.; Barralet, J. E.
Journal: Acta Biomater (2015): 140-6

Visualizing Lysosomal Membrane Permeabilization by Fluorescent Dextran Release
Authors: Ellegaard, A. M.; Jaattela, M.; Nyl and sted, J.
Journal: Cold Spring Harb Protoc (2015): 900-3

Pulmonary permeability assessed by fluorescent-labeled dextran instilled intranasally into mice with LPS-induced acute lung injury
Authors: Chen, H.; Wu, S.; Lu, R.; Zhang, Y. G.; Zheng, Y.; Sun, J.
Journal: PLoS One (2014): e101925

Selective fluorescent detection of aspartic acid and glutamic acid employing dansyl hydrazine dextran conjugate
Authors: Nasomphan, W.; Tangboriboonrat, P.; Tanapongpipat, S.; Smanmoo, S.
Journal: J Fluoresc (2014): 11-Jul

Dextran-based fluorescent nanoprobes for sentinel lymph node mapping
Authors: Dai, T.; Zhou, S.; Yin, C.; Li, S.; Cao, W.; Liu, W.; Sun, K.; Dou, H.; Cao, Y.; Zhou, G.
Journal: Biomaterials (2014): 8227-35

Facile fabrication of dextran-based fluorescent nanogels as potential glucose sensors
Authors: Zhou, S.; Min, X.; Dou, H.; Sun, K.; Chen, C. Y.; Chen, C. T.; Zhang, Z.; Jin, Y.; Shen, Z.
Journal: Chem Commun (Camb) (2013): 9473-5

Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays
Authors: Egea, A. M.; Trevisiol, E.; Vieu, C.
Journal: Biointerphases (2013): 37