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Cell Navigator® Lysosome Staining Kit
Green Fluorescence with 405 nm Excitation
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label lysosomes of live cells in green fluorescence. The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably vial the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly increases its selectivity for lysosomes. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. This kit is optimized for flow cytometers equiped with viloet laser excitation @ 405 nm and emission around 500 nm.
Image of U2OS cells stained with Cell Navigator® Lysosomal Staining Kit in a Costar black wall/clear bottom 96-well plate.
Image of U2OS cells stained with Cell Navigator® Lysosomal Staining Kit in a Costar black wall/clear bottom 96-well plate.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22651500 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation405 nm
Emission480 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Violet filter set
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Page updated on September 20, 2025