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iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 647 PSA™ kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with rabbit anti-EpCaM antibody and then incubated with poly HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 647 Styramide™ (Cat#45240) or Alexa Fluor® 647 tyramide stain respectively. Images showed that iFluor® 647 PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 647 TSA method.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with rabbit anti-EpCaM antibody and then incubated with poly HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 647 Styramide™ (Cat#45240) or Alexa Fluor® 647 tyramide stain respectively. Images showed that iFluor® 647 PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 647 TSA method.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
45240100 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.03
Correction factor (280 nm)0.03
Correction factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)656
Emission (nm)670
Quantum yield
0.25
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Power Styramide™ Signal Amplification (PSA™)
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Page updated on December 21, 2025