Name: mFluor™ Violet 430 SE
Brand: AAT Bioquest
SKU: 1148
Price: 237 USD
Availability: InStock

mFluor™ Violet 430 SE

mFluor™ Violet 430 SE is a violet laser-optimized NHS ester dye that enables bright antibody labeling with minimal spectral overlap, designed for high-parameter flow cytometry and spectral cytrometry applications.

Reactive Moiety: Succinimidyl ester enables efficient, covalent attachment to primary amines on proteins and antibodies
Laser Compatibility: Optimally excited by the 405 nm violet laser
High signal-to-noise ratio: Narrow emission profile with low spillover into adjacent channels, facilitating complex multicolor panel design
Application: Ideal for covalent labeling of antibodies, proteins, and other amine-containing biomolecules for flow cytometric analysis

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with mFluor™ Violet 430 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 430 specific V1-A channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1148	1 mg	Price

Overview

mFluor™ Violet 430 SE is a succinimidyl ester-activated fluorescent dye specifically designed for covalent labeling of proteins, including antibodies, via primary amines. With an excitation maximum at 411 nm and an emission maximum at 426 nm, it is optimally excited by the 405 nm violet laser and is ideally suited for use in multicolor flow cytometry panels. The dye exhibits high fluorescence intensity and minimal spectral spillover, enabling precise discrimination in multiplexed assays. Conjugates prepared with mFluor™ Violet 430 SE are stable and compatible with standard fixation and permeabilization protocols, making the dye suitable for both surface and intracellular staining applications in flow cytometric analysis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 430 SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.082 µL	610.411 µL	1.221 mL	6.104 mL	12.208 mL
5 mM	24.416 µL	122.082 µL	244.164 µL	1.221 mL	2.442 mL
10 mM	12.208 µL	61.041 µL	122.082 µL	610.411 µL	1.221 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	819.12 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.

Authors:

van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G

Journal:

Journal of fluorescence (2021): 835-845

Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.

Authors:

Abbas, Ibrahim and Hildreth, Michael

Journal:

Veterinary parasitology (2019): 69-74

Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.

Authors:

Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi

Journal:

Nature structural & molecular biology (2019): 526

Identification of novel cell-impermeant fluorescent substrates for testing the function and drug interaction of Organic Anion-Transporting Polypeptides, OATP1B1/1B3 and 2B1.

Authors:

Patik, Izabel and Székely, Virág and Német, Orsolya and Szepesi, Áron and Kucsma, Nóra and Várady, György and Szakács, Gergely and Bakos, Éva and Özvegy-Laczka, Csilla

Journal:

Scientific reports (2018): 2630

Energy transfer between a biological labelling dye and gold nanorods.

Authors:

Racknor, Chris and Singh, Mahi R and Zhang, Yinan and Birch, David J S and Chen, Yu

Journal:

Methods and applications in fluorescence (2013): 015002

Physical properties

Molecular weight	819.12
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.136
Correction factor (280 nm)	0.092
Extinction coefficient (cm ^-1 M ^-1)	60000 ¹
Excitation (nm)	410
Emission (nm)	424

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 9, 2026