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mFluor™ Violet 430 SE

Product key features

  • Excitation/Emission: 411 nm / 426 nm.
  • Reactive Moiety: Succinimidyl ester enables efficient, covalent attachment to primary amines on proteins and antibodies.
  • Laser Compatibility: Optimally excited by the 405 nm violet laser.
  • High signal-to-noise ratio: Narrow emission profile with low spillover into adjacent channels, facilitating complex multicolor panel design.
  • Application: Ideal for covalent labeling of antibodies, proteins, and other amine-containing biomolecules for flow cytometric analysis.

Product description

mFluor™ Violet 430 SE is a succinimidyl ester-activated fluorescent dye specifically designed for covalent labeling of proteins, including antibodies, via primary amines. With an excitation maximum at 411 nm and an emission maximum at 426 nm, it is optimally excited by the 405 nm violet laser and is ideally suited for use in multicolor flow cytometry panels. The dye exhibits high fluorescence intensity and minimal spectral spillover, enabling precise discrimination in multiplexed assays. Conjugates prepared with mFluor™ Violet 430 SE are stable and compatible with standard fixation and permeabilization protocols, making the dye suitable for both surface and intracellular staining applications in flow cytometric analysis.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 450 SE4064453500010.8110.3380.078
mFluor™ Violet 480 SE40447540,0000.62511.230.6570
mFluor™ Violet 500 SE4105012500010.8110.7690.365
mFluor™ Violet 505 SE3935044000010.4510.8880.403
mFluor™ Violet 510 SE4125052500010.8610.4640.366
mFluor™ Violet 530 SE505525200001---
mFluor™ Violet 540 SE4025351800010.2111.3260.543
mFluor™ Violet 545 SE3935432000010.1511.080.496
mFluor™ Violet 550 SE5275509000010.3110.4740.306
mFluor™ Violet 590 SE5645919000010.2210.6320.329
mFluor™ Violet 610 SE5966129000010.310.5320.66
Show More (2)

References

View all 6 references: Citation Explorer
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Identification of novel cell-impermeant fluorescent substrates for testing the function and drug interaction of Organic Anion-Transporting Polypeptides, OATP1B1/1B3 and 2B1.
Authors: Patik, Izabel and Székely, Virág and Német, Orsolya and Szepesi, Áron and Kucsma, Nóra and Várady, György and Szakács, Gergely and Bakos, Éva and Özvegy-Laczka, Csilla
Journal: Scientific reports (2018): 2630
Energy transfer between a biological labelling dye and gold nanorods.
Authors: Racknor, Chris and Singh, Mahi R and Zhang, Yinan and Birch, David J S and Chen, Yu
Journal: Methods and applications in fluorescence (2013): 015002
Page updated on May 19, 2025

Ordering information

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Catalog Number1148
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Request quotation

Physical properties

Molecular weight

708.77

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.17

Extinction coefficient (cm -1 M -1)

700001

Excitation (nm)

411

Emission (nm)

426

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with mFluor™ Violet 430 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 430 specific V1-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with mFluor™ Violet 430 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 430 specific V1-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with mFluor™ Violet 430 anti-human CD4 *RPA-T4* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 430 specific V1-A channel.
Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.

Documents

pdfSafety data sheet
pdfCertificate of analysis
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